{"id":4649,"date":"2021-12-07T07:54:18","date_gmt":"2021-12-07T07:54:18","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=4649"},"modified":"2023-01-20T00:31:49","modified_gmt":"2023-01-20T00:31:49","slug":"microscopic-particle-analysis","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/microscopic-particle-analysis\/","title":{"rendered":"Microscopic Particle Analysis"},"content":{"rendered":"\r\n<h1>Microscopy Particle Size Analysis<\/h1>\r\n<p>Characterize particle count, size, and shape<\/p>\r\n<ul>\r\n<li><a href=\"#tech\">Tech<\/a><\/li>\r\n<li><a href=\"#hound\">Hound<\/a><\/li>\r\n<\/ul>\r\n<h3>Why the big fuss over these little particles?<\/h3>\r\n<p>Particulate contamination in injectable drug products can cause undesired immune responses, vascular blockages, and a range of other complications. Because of this concern each vial, syringe, or container of a drug product must be inspected for visible particles. One of the microscopic methods for particle size determination is membrane microscopy.<\/p>\r\n<p>Some drug products intentionally have particles, such as inhalers, topical creams, pills and tablets, and lyophilized products. In these cases, light microscopy can aid particle characterization by providing counts of different particle types or analyzing the size distribution of particles present.<\/p>\r\n<p>Whether particles are part of the product or an unwanted contaminant, microscopy lets you quickly determine particle count, size, and shape for visible and subvisible particles in liquids, powders, creams, and other materials.<\/p>\r\n<h3>Prep those particles<\/h3>\r\n<p>Particles can be isolated from the drug product by membrane filtration. Gold-coated filter rounds maximize contrast between the particles and background, so no particles are missed.\u00a0Amorphous particles can be analyzed in suspension. Thanks to the flexibility of microscopy, imaging and counting particles in a liquid suspension is no problem.\u00a0Powders and creams can be spread in a monolayer for analysis.<\/p>\r\n<p><img loading=\"lazy\" decoding=\"async\" title=\"particle_capture-2\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/particle_capture-2.jpg\" alt=\"particle_capture-2\" width=\"635\" height=\"557\" \/><\/p>\r\n<h3>Make it count<\/h3>\r\n<p>A series of images are collected and stitched together, creating a mosaic image spanning a large area. A predefined threshold can be used to differentiate particles versus background, resulting in a particle count for the sample.<\/p>\r\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Make it count_less small\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/Make-it-count_less-small.jpg\" alt=\"Make it count_less small\" width=\"1013\" height=\"938\" \/><\/p>\r\n<h3>Size them up<\/h3>\r\n<p>With images collected of each particle, the size and shape can be fully characterized. Automated microscopy measures each dimension of a particle and calculates various morphological parameters, such as elongation, fibrosity, or equivalent circle diameter.<\/p>\r\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Size them up\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/Size-them-up.jpg\" alt=\"Size them up\" width=\"1237\" height=\"994\" \/> <img loading=\"lazy\" decoding=\"async\" title=\"dark_field\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/dark_field.jpg\" alt=\"dark_field\" width=\"1000\" height=\"478\" \/><\/p>\r\n<h3>Get organized<\/h3>\r\n<p>Particles can be binned based on size or other morphological parameters, so you can go back and focus on the particles that matter the most.<\/p>\r\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Get Organized_r3\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Get-Organized_r3.png\" alt=\"Get Organized_r3\" width=\"2122\" height=\"1365\" \/> <img decoding=\"async\" title=\"Hound_front_open_transparent_shadow\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/Hound_front_open_transparent_shadow-1.jpg\" alt=\"Hound_front_open_transparent_shadow\" \/><\/p>\r\n<h3>Hound<\/h3>\r\n<p>Combining automated microscopy,<a href=\"https:\/\/unchainedlabs.com\/jp\/raman-microscopy\/\"> Raman spectroscopy<\/a>, and <a href=\"https:\/\/unchainedlabs.com\/jp\/laser-induced-spectroscopy-libs\/\">Laser Induced Breakdown Spectroscopy<\/a> (LIBS) in a single instrument, Hound enables manual and automated identification of visible and subvisible particles across a wide range of chemical compositions.<\/p>\r\n<p><a role=\"button\" href=\"https:\/\/unchainedlabs.com\/jp\/hound\/\" target=\"_self\" rel=\"noopener\" aria-label=\"Meet Hound\"> Meet Hound <\/a><\/p>\r\n<h3>Ready for more?<\/h3>\r\n<p>Biopharma scientists can now use the right tool for microscopy particle size analysis and identification challenges with an instrument that combines laser induced breakdown spectroscopy, Raman spectroscopy, and light microscopy. Have a question or ready to find out more? Here&#8217;s the best way to reach us:<\/p>\r\n<p><a role=\"button\" href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\/\" target=\"_self\" rel=\"noopener\"> Get In touch <\/a><\/p>\r\n","protected":false},"excerpt":{"rendered":"<p>Microscopy Particle Size Analysis Characterize particle count, size, and shape Tech Hound Why the big fuss over these little particles? Particulate contamination in injectable drug products can cause undesired immune responses, vascular blockages, and a range of other complications. Because of this concern each vial, syringe, or container of a drug product must be inspected&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-4649","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Microscopy Particle Size Analysis | Unchained Labs<\/title>\n<meta name=\"description\" content=\"Learn how light microscopy can aid particle characterization by providing counts of different particle types or analyzing the size distribution of the particles.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/unchainedlabs.com\/jp\/microscopic-particle-analysis\/\" 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