{"id":4652,"date":"2021-12-07T07:58:12","date_gmt":"2021-12-07T07:58:12","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=4652"},"modified":"2024-05-17T04:49:26","modified_gmt":"2024-05-17T04:49:26","slug":"quantification-aggregation","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/quantification-aggregation\/","title":{"rendered":"Protein aggregation &#038; quantification"},"content":{"rendered":"\n<h1>\n\t\t\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u51dd\u96c6\uff06\u5b9a\u91cf\n\t<\/h1>\n\t<ul>\n<li><a href=\"#problem\">\u8ab2\u984c<\/a><\/li>\n<li><a href=\"#solution\">\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/a><\/li>\n<li><a href=\"#proof\">\u5b9f\u8a3c\u30c7\u30fc\u30bf<\/a><\/li>\n<li><a href=\"#stunner\">\u95a2\u9023\u88fd\u54c1 <strong>&#8211;<\/strong> <strong>Stunner<\/strong><\/a><\/li>\n<\/ul>\n<h4>\n\t\t\u8ab2\u984c\n\t<\/h4>\n\t<p>\u65b0\u3057\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7814\u7a76\u3057\u59cb\u3081\u305f\u521d\u671f\u6bb5\u968e\u3067\u306f\u3001\u305d\u306e\u30b5\u30f3\u30d7\u30eb\u304c\u88fd\u5264\u5316\u306b\u8010\u3048\u3046\u308b\u54c1\u8cea\u304b\u3069\u3046\u304b\u306f\u307e\u3060\u308f\u304b\u308a\u307e\u305b\u3093\u3002\u53ce\u91cf\u304c\u4ee5\u524d\u3068\u9055\u3063\u3066\u3044\u308b\u5834\u5408\u3084\u7cbe\u88fd\u30d7\u30ed\u30bb\u30b9\u304c\u6700\u9069\u5316\u3055\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u3082\u3088\u304f\u3042\u308a\u307e\u3059\u3002\u51dd\u96c6\u4f53\u304c\u6f5c\u3093\u3067\u3044\u3066\u3001\u6b21\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u53f0\u7121\u3057\u306b\u3059\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u4f7f\u7528\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u306e\u91cf\u3084\u51dd\u96c6\u306e\u6709\u7121\u3092\u77e5\u308b\u3053\u3068\u306f\u3001\u7279\u6027\u89e3\u6790\u3084\u30a2\u30c3\u30bb\u30a4\u958b\u767a\u7814\u7a76\u3092\u958b\u59cb\u3059\u308b\u524d\u306b\u3001\u6642\u9593\u3068\u8cb4\u91cd\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u7bc0\u7d04\u3059\u308b\u306e\u306b\u5fc5\u8981\u306a\u30c7\u30fc\u30bf\u3067\u3059\u3002<\/p>\n<h4>\n\t\t\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\n\t<\/h4>\n\t<p><a href=\"https:\/\/www.unchainedlabs.com\/jp\/stunner\">Stunner<\/a>\u306f\u3001\u6fc3\u5ea6\u6e2c\u5b9a\u306e\u305f\u3081\u306e\u9ad8\u901f<a href=\"https:\/\/www.unchainedlabs.com\/jp\/uv-vis-spectroscopy\">UV\/Vis<\/a>\u5206\u6790\u3068\u3001\u30b5\u30a4\u30ba\u6e2c\u5b9a\u3084\u51dd\u96c6\u4f53\u63a2\u7d22\u306e\u305f\u3081\u306e<a href=\"https:\/\/www.unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">\u56de\u8ee2\u89d2\u52d5\u7684\u5149\u6563\u4e71<\/a>\uff08RADLS\uff09\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8a73\u7d30\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Stunner\u306f1\u30b5\u30f3\u30d7\u30eb\u3042\u305f\u308a2\u00b5L\u3092\u4f7f\u7528\u3057\u3001UV\/Vis\u3068RADLS\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u6790\u306e\u4e21\u65b9\u3067\u6700\u592796\u30b5\u30f3\u30d7\u30eb\u3092\u7d042\u6642\u9593\u3067\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u3068\u540c\u6642\u306b\u3001Stunner\u306fRADLS\u3092\u4f7f\u7528\u3057\u3066\u30b5\u30f3\u30d7\u30eb\u306e\u6d41\u4f53\u529b\u5b66\u7684\u76f4\u5f84\u3092\u6e2c\u5b9a\u3057\u3001\u51dd\u96c6\u304c\u554f\u984c\u306b\u306a\u308b\u304b\u3069\u3046\u304b\u3092\u7279\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Stunner\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8a73\u7d30\u3092\u8aad\u307f\u53d6\u308c\u3070\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u72b6\u614b\u3092\u6b63\u78ba\u306b\u628a\u63e1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2024\/01\/stunner_workflow_Biologics_web.png\" alt=\"stunner_workflow_Biologics_web\" itemprop=\"image\" height=\"550\" width=\"821\" title=\"stunner_workflow_Biologics_web\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\t\u5b9f\u8a3c\u30c7\u30fc\u30bf\n\t<\/h4>\n<h3>\n\t\t\u540c\u6642\u306b\u5b9f\u884c\u3067\u304d\u308b\u5b9a\u91cf\u3068\u30b5\u30a4\u30b8\u30f3\u30b0\n\t<\/h3>\n\t<p>\u540c\u4e00\u30b5\u30f3\u30d7\u30eb\u304b\u3089<a href=\"https:\/\/www.unchainedlabs.com\/jp\/uv-vis-spectroscopy\/\">UV\/Vis<\/a>\u3068<a href=\"https:\/\/www.unchainedlabs.com\/jp\/dynamic-light-scattering\/\">DLS<\/a>\u3092\u8aad\u307f\u53d6\u308b\u3053\u3068\u3067\u3001\u6d41\u4f53\u529b\u5b66\u7684\u76f4\u5f84\u306b\u3088\u308b\u51dd\u96c6\u306e\u6709\u7121\u3001\u591a\u5206\u6563\u6027\u304b\u3089\u5747\u4e00\u6027\u3092\u30c1\u30a7\u30c3\u30af\u3057\u3001\u6b63\u78ba\u306a\u6fc3\u5ea6\u3092\u4e00\u5ea6\u306b\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u9ad8\u6fc3\u5ea6\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u5de8\u5927\u306a\u51dd\u96c6\u4f53\u304c\u6df7\u3056\u3063\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u6700\u9069\u306a\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u306a\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/DLS_combo-1.png\" alt=\"DLS_combo-1\" itemprop=\"image\" height=\"395\" width=\"450\" title=\"DLS_combo-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/DLS_combo-2.png\" alt=\"DLS_combo-2\" itemprop=\"image\" height=\"378\" width=\"450\" title=\"DLS_combo-2\" onerror=\"this.style.display='none'\"  \/>\n<h3>\n\t\t\u601d\u3044\u901a\u308a\u306e\u6e2c\u5b9a\n\t<\/h3>\n\t<p><a href=\"https:\/\/www.unchainedlabs.com\/jp\/stunner\">Stunner\u306eUV\/Vis<\/a>\u306b\u3088\u308b<a href=\"https:\/\/www.unchainedlabs.com\/jp\/protein-quantification\/\">\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf<\/a>\u306f\u3001\u8a66\u85ac\u3084\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3092\u5fc5\u8981\u3068\u3057\u307e\u305b\u3093\u30020.02mg\/mL\u304b\u3089200mg\/mL(mAb)\u307e\u3067\u306e\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u30ec\u30f3\u30b8\u304c\u3042\u308a\u3001\u3069\u3093\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3082\u5e0c\u91c8\u306a\u3057\u3067\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002Stunner\u306f2\u3064\u306e\u56fa\u5b9a\u5149\u8def\u9577\u306b\u3088\u3063\u3066\u30012%\u4ee5\u5185\u306e\u6b63\u78ba\u6027\u30681%\u4ee5\u5185\u306e\u7cbe\u5ea6\u3067\u3001\u5168\u6fc3\u5ea6\u7bc4\u56f2\u306b\u308f\u305f\u3063\u3066\u7684\u78ba\u306a\u30c7\u30fc\u30bf\u3092\u63d0\u4f9b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Max-out_trans-450t-1.jpg\" alt=\"Max-out_trans-450t-1\" itemprop=\"image\" height=\"439\" width=\"450\" title=\"Max-out_trans-450t-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Max-out_trans-450t-2.jpg\" alt=\"Max-out_trans-450t-2\" itemprop=\"image\" height=\"434\" width=\"450\" title=\"Max-out_trans-450t-2\" onerror=\"this.style.display='none'\"  \/>\n<h3>\n\t\t\u4e0d\u7d14\u7269\u306e\u30c1\u30a7\u30c3\u30af\n\t<\/h3>\n\t<p>\u51dd\u96c6\u4f53\u3092\u30c1\u30a7\u30c3\u30af\u3059\u308b\u3064\u3044\u3067\u306b\u3001\u4e0d\u7d14\u7269\u3082\u30c1\u30a7\u30c3\u30af\u3057\u3066\u307f\u307e\u305b\u3093\u304b\u3002Stunner\u306e<a href=\"https:\/\/www.unchainedlabs.com\/jp\/uv-vis-spectroscopy\">UV\/Vis<\/a>\u306b\u306fUnmix\u3068\u3044\u3046\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u304c\u642d\u8f09\u3055\u308c\u3066\u304a\u308a\u3001\u5438\u5149\u5ea6\u30b9\u30da\u30af\u30c8\u30eb\u3092\u5206\u89e3\u3057\u3066\u3001\u305d\u3053\u306b\u4f55\u304c\u6f5c\u3093\u3067\u3044\u308b\u304b\u3092\u8abf\u3079\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002A280\u304c\u6fc1\u308a\u3001\u6838\u9178\u3001EDTA\u3001\u305d\u306e\u4ed6\u306e\u4e0d\u7d14\u7269\u306e\u5f71\u97ff\u3092\u53d7\u3051\u3066\u3044\u308b\u304b\u3069\u3046\u304b\u3092\u78ba\u8a8d\u3057\u3001\u305d\u306e\u3088\u3046\u306a\u554f\u984c\u304c\u3042\u3063\u3066\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/See-whats-in-there_r2.png\" alt=\"See what&#039;s in there_r2\" itemprop=\"image\" height=\"1375\" width=\"2145\" title=\"See what&#039;s in there_r2\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Stunner-Front-cc_crop_downsize.png\" alt=\"Stunner Front cc_crop_downsize\" title=\"Stunner Front cc_crop_downsize\" itemprop=\"image\"\/>\n\t\t\t<h3>Stunner<\/h3>\t\t\t\n\t\t\t\t<p>Stunner\u306f\u3001\u540c\u30582\u03bcL\u306e\u30b5\u30f3\u30d7\u30eb\u304b\u3089<a href=\"https:\/\/www.unchainedlabs.com\/jp\/uv-vis-spectroscopy\">UV\/Vis<\/a>\u6fc3\u5ea6\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/dynamic-light-scattering\/\">\u52d5\u7684\u5149\u6563\u4e71<\/a>\uff08DLS\uff09\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/static-light-scattering\/\">\u9759\u7684\u5149\u6563\u4e71<\/a>\uff08SLS\uff09\u30c7\u30fc\u30bf\u3092\u5f15\u304d\u51fa\u305b\u308b\u552f\u4e00\u306e\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u30d9\u30fc\u30b9\u3067\u30de\u30eb\u30c1\u30c1\u30e3\u30f3\u30cd\u30eb\u306e\u30d4\u30da\u30c3\u30c8\u3092\u5099\u3048\u3001\u81ea\u52d5\u5316\u4f5c\u696d\u5bfe\u5fdc\u3067\u3001\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u304b\u3064\u9ad8\u6fc3\u5ea6\u3067\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u3092\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002\u6fc3\u5ea6\u3001\u6d41\u4f53\u529b\u5b66\u7684\u534a\u5f84\u3001\u591a\u5206\u6563\u6027\u3001\u51dd\u96c6\u4f53\u306e\u691c\u51fa\u3092\u4e00\u62ec\u3067\u5b8c\u6210\u3067\u304d\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u54c1\u8cea\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u554f\u984c\u306a\u3044\u304b\u3069\u3046\u304b\u3001\u3059\u3050\u306b\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/stunner\" target=\"_self\" role=\"button\" aria-label=\"STUNNER\u95a2\u9023\u60c5\u5831\" rel=\"noopener\">\n\t\t\t\t\t\t\tSTUNNER\u95a2\u9023\u60c5\u5831\n\t\t<\/a>\n<h2>\n\t\t\u3055\u3089\u306b\u8a73\u3057\u3044\u60c5\u5831\u306f\u3053\u3061\u3089\n\t<\/h2>\n\t<p>\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u3092\u7c21\u4fbf\u306b\u6e2c\u5b9a\u3067\u304d\u308bStunner\u306b\u3064\u3044\u3066\u3001\u3082\u3063\u3068\u77e5\u308a\u305f\u3044\u3067\u3059\u304b\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u304a\u554f\u3044\u5408\u308f\u305b\n\t\t\t\t\t<\/a>\n<h2>\n\t\t\u95a2\u9023\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\n\t<\/h2>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/stability-formulation-screening\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u5b89\u5b9a\u6027\uff06\u88fd\u5264\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\n\t\t\t\t\t<\/a>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/buffer-exchange-and-sample-preparation\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\uff06\u30b5\u30f3\u30d7\u30eb\u6e96\u5099\n\t\t\t\t\t<\/a>\n\n","protected":false},"excerpt":{"rendered":"<p>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u51dd\u96c6\uff06\u5b9a\u91cf \u8ab2\u984c \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 \u5b9f\u8a3c\u30c7\u30fc\u30bf \u95a2\u9023\u88fd\u54c1 &#8211; Stunner \u8ab2\u984c 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