{"id":4820,"date":"2021-12-07T12:24:55","date_gmt":"2021-12-07T12:24:55","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=4820"},"modified":"2026-10-19T01:18:19","modified_gmt":"2026-10-19T01:18:19","slug":"dynamic-light-scattering","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/dynamic-light-scattering\/","title":{"rendered":"Dynamic Light Scattering (DLS)"},"content":{"rendered":"\n<h1>\n\t\t\t\u52d5\u7684\u5149\u6563\u4e71\u6cd5\uff08DLS\uff09\u3068\u306f\uff5c\u539f\u7406\u3068\u7c92\u5b50\u5f84\u30fb\u51dd\u96c6\u306e\u6e2c\u5b9a\t<\/h1>\n\t<p>\u30bf\u30f3\u30d1\u30af\u8cea\u30fb\u305d\u306e\u4ed6\u306e\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u52d5\u7684\u5149\u6563\u4e71\u6e2c\u5b9a<\/p>\n\t<ul>\n<li><a href=\"#tech\">\u6280\u8853<\/a><\/li>\n<li><a href=\"#stunner\">\u95a2\u9023\u88fd\u54c1<\/a><strong><a href=\"#stunner\"> &#8211; Stunner<\/a><\/strong><\/li>\n<li><a href=\"#aunty\">\u95a2\u9023\u88fd\u54c1<\/a><strong><a href=\"#aunty\"> &#8211; Aunty<\/a><\/strong><\/li>\n<li><strong><a href=\"#faq\">FAQ<\/a><\/strong><\/li>\n<\/ul>\n<h5>\n\t\t\t\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u7814\u7a76\u958b\u767a\u8005\u5411\u3051\u52d5\u7684\u5149\u6563\u4e71\u88c5\u7f6e\t<\/h5>\n\t<p>\u52d5\u7684\u5149\u6563\u4e71\uff08DLS\uff09\u6cd5\u306f\u3001\u6eb6\u6db2\u4e2d\u306e\u5206\u5b50\u3084\u7c92\u5b50\u306b\u3088\u3063\u3066\u6563\u4e71\u3055\u308c\u308b\u30ec\u30fc\u30b6\u30fc\u5149\u5f37\u5ea6\u306e\u6025\u6fc0\u306a\u5909\u5316\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u7c92\u5b50\u5f84\u3068\u7c92\u5b50\u5f84\u5206\u5e03\u3092\u628a\u63e1\u3059\u308b\u6280\u8853\u3067\u3059\u3002DLS\u5206\u6790\u306f\u3001\u30da\u30d7\u30c1\u30c9\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30a6\u30a4\u30eb\u30b9\u3001VLP\u306a\u3069\u3001\u591a\u304f\u306e\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u30b5\u30a4\u30ba\u3092\u7d20\u65e9\u304f\u3001\u30e9\u30d9\u30eb\u30d5\u30ea\u30fc\u3067\u3001\u975e\u7834\u58ca\u7684\u306b\u628a\u63e1\u3059\u308b\u65b9\u6cd5\u3067\u3059\u300210nm\u306e\u6297\u4f53\u300130nm\u306eAAV\u3001\u307e\u305f\u306f\u30b5\u30d6\u30df\u30af\u30ed\u30f3\u30b5\u30a4\u30ba\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u306e\u5e73\u5747\u30b5\u30a4\u30ba\u3068\u51dd\u96c6\u72b6\u614b\u3092\u8aad\u307f\u53d6\u308b\u306b\u306f\u3001\u751f\u7269\u88fd\u5264\u7814\u7a76\u8005\u306b\u3068\u3063\u3066\u6700\u826f\u306e\u9078\u629e\u80a2\u3067\u3059\u3002\u307e\u305f\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">\u56de\u8ee2\u89d2\u52d5\u7684\u5149\u6563\u4e71<\/a>\uff08RADLS\uff09\u6cd5\u306f\u3001\u8907\u6570\u306e\u89d2\u5ea6\u304b\u3089\u30c7\u30fc\u30bf\u3092\u53ce\u96c6\u3059\u308b\u3053\u3068\u3067\u3001DLS\u3092\u57fa\u790e\u3068\u3059\u308b\u3055\u3089\u306b\u5f37\u529b\u306a\u6280\u8853\u3067\u3059\u3002<\/p>\n<p>Unchained Labs\u306b\u306f\u3001\u554f\u984c\u89e3\u6c7a\u306e\u305f\u3081\u306e\u7a76\u6975\u306eDLS\u88c5\u7f6e\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/stunner\/\">Stunner<\/a>\u3068<a href=\"https:\/\/www.unchainedlabs.com\/jp\/stunner\/\">Aunty<\/a>\u304c\u3042\u308a\u307e\u3059\u3002Stunner\u3068Aunty\u306f\u3001\u3069\u3061\u3089\u3082\u5fae\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u7a3c\u50cd\u3067\u304d\u307e\u3059\u304c\u3001\u5404\u88c5\u7f6e\u306b\u305d\u308c\u305e\u308c\u306e\u7279\u9577\u304c\u3042\u308a\u307e\u3059\u3002\u6fc3\u5ea6\u3068\u30b5\u30a4\u30ba\u3092\u3059\u3070\u3084\u304f\u6e2c\u5b9a\u3057\u305f\u3051\u308c\u3070Stunner\u3092\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5b89\u5b9a\u6027\u306e\u5168\u4f53\u50cf\u3092\u628a\u63e1\u3057\u305f\u3051\u308c\u3070\u3001Aunty\u304c\u305d\u308c\u305e\u308c\u306e\u76ee\u7684\u306b\u9069\u3057\u305f\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002<\/p>\n<h5>\n\t\t\t\u52d5\u7684\u5149\u6563\u4e71\u6cd5\u306e\u539f\u7406\t<\/h5>\n\t<p>&nbsp;<\/p>\n<p>\u7c92\u5b50\u306e\u6eb6\u6db2\u306b\u30ec\u30fc\u30b6\u30fc\u5149\u3092\u7167\u5c04\u3059\u308b\u3068\u3001\u591a\u304f\u306e\u6563\u4e71\u5149\u3092\u691c\u51fa\u3067\u304d\u307e\u3059\u3002\u30ec\u30fc\u30b6\u30fc\u306e\u6ce2\u9577\u304c\u7c92\u5b50\u30b5\u30a4\u30ba\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u5927\u304d\u3044\u3068\u3001\u3069\u306e\u65b9\u5411\u306b\u3082\u540c\u3058\u91cf\u306e\u5149\u304c\u62e1\u6563\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001DLS\u6a5f\u5668\u306b\u306f660nm\u30ec\u30fc\u30b6\u30fc\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>DLS\u306f\u3001\u6563\u4e71\u5f37\u5ea6\u304c\u6642\u9593\u306e\u7d4c\u904e\u3068\u5171\u306b\u3069\u308c\u3060\u3051\u6025\u901f\u306b\u5909\u5316\u3059\u308b\u304b\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u6eb6\u6db2\u4e2d\u306e\u7c92\u5b50\u306e\u30b5\u30a4\u30ba\u306b\u3064\u3044\u3066\u591a\u304f\u306e\u3053\u3068\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u56f31\uff09\u3002\u5c0f\u3055\u306a\u7c92\u5b50\u306f\u7d20\u65e9\u304f\u52d5\u304d\u56de\u308b\u306e\u3067\u3001\u5149\u306e\u5f37\u5ea6\u306f\u7d20\u65e9\u304f\u5909\u5316\u3057\u307e\u3059\u3002\u5927\u304d\u306a\u7c92\u5b50\u306f\u52d5\u304d\u56de\u308b\u306e\u304c\u9045\u3044\u306e\u3067\u3001\u305d\u306e\u9006\u3067\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u5149\u5f37\u5ea6\u306e\u5909\u5316\u304c\u901f\u3044\u304b\u9045\u3044\u304b\u3092\u5206\u6790\u3059\u308b\u306e\u304cDLS\u5206\u6790\u3067\u3059\u3002<\/p>\n<p>DLS\u306e\u5149\u6563\u4e71\u30c7\u30fc\u30bf\u306e\u5206\u6790\u306b\u3064\u3044\u3066\u8003\u3048\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u3042\u308b\u6642\u70b9\u3092\u9078\u629e\u3057\u30011\u30de\u30a4\u30af\u30ed\u79d2\u65e9\u9001\u308a\u3057\u307e\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u7c92\u5b50\u306f\u307e\u3060\u52d5\u304d\u56de\u3063\u3066\u3044\u306a\u3044\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u306e\u3067\u3001\u5149\u6563\u4e71\u306f\u5909\u5316\u3057\u3066\u304a\u3089\u305a\u3001\u305d\u306e\u30bc\u30ed\u6642\u70b9\u306e\u30c7\u30fc\u30bf\u30681\u30de\u30a4\u30af\u30ed\u79d2\u5f8c\u306e\u30c7\u30fc\u30bf\u306f\u307b\u307c\u540c\u3058\u3067\u3059\u3002\u3064\u307e\u308a\u3001\u9ad8\u3044\u76f8\u95a2\u95a2\u4fc2\u304c\u3042\u308b\u306e\u3067\u3059\u3002<\/p>\n<p>\u6b21\u306f\uff11\u30de\u30a4\u30af\u30ed\u79d2\u306e\u4ee3\u308f\u308a\u306b\u30011\u79d2\u5f8c\u306e\u30c7\u30fc\u30bf\u3092\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u307b\u3068\u3093\u3069\u306e\u7c92\u5b50\u306f\u307e\u3063\u305f\u304f\u9055\u3046\u5834\u6240\u306b\u3042\u308a\u3001\u958b\u59cb\u70b9\u30681\u79d2\u5f8c\u306e\u9593\u306e\u30c7\u30fc\u30bf\u306e\u76f8\u95a2\u306f\u30bc\u30ed\u306b\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u76f8\u95a2\u5024\u3092\u3055\u307e\u3056\u307e\u306a\u6642\u9593\u306e\u7d4c\u904e\u306b\u3064\u3044\u3066\u30b0\u30e9\u30d5\u306b\u3059\u308b\u3068\u3001\u76f8\u95a2\u95a2\u6570\u304c\u5f97\u3089\u308c\u307e\u3059\uff08\u56f32\uff09\u3002\u305d\u3057\u3066\u3001\u76f8\u95a2\u304c\u9ad8\u3044\u72b6\u614b\u304b\u3089\u76f8\u95a2\u304c\u30bc\u30ed\u306b\u306a\u308b\u307e\u3067\u306e\u901f\u3055\u304b\u3089\u3001\u7c92\u5b50\u306e\u5e73\u5747\u30b5\u30a4\u30ba\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u52d5\u7684\u5149\u6563\u4e71\u304c\u5149\u5b50\u76f8\u95a2\u5206\u5149\u6cd5\uff08PCS\uff09\u3068\u547c\u3070\u308c\u308b\u7406\u7531\u3067\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u76f8\u95a2\u95a2\u6570\u304b\u3089\u3001\u672c\u5f53\u306b\u5f97\u305f\u3044\u30c7\u30fc\u30bf\u306b\u305f\u3069\u308a\u7740\u304f\u306b\u306f\u30012\u3064\u306e\u5206\u6790\u65b9\u6cd5\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002\u4e00\u3064\u76ee\u306e\u65b9\u6cd5\u306f\u3001\u76f8\u95a2\u95a2\u6570\u306b\u300c\u30d9\u30b9\u30c8\u30d5\u30a3\u30c3\u30c8\u300d\u3092\u9069\u7528\u3057\u3001\u305d\u306e\u30d5\u30a3\u30c3\u30c8\u306e\u5f62\u72b6\u304b\u3089\u62e1\u6563\u4fc2\u6570\u3001\u5e73\u5747\u7c92\u5b50\u5f84\u3001\u7c92\u5b50\u5f84\u5206\u5e03\u3092\u6c42\u3081\u308b\u306e\u3067\u3059\u3002\u62e1\u6563\u4fc2\u6570\u304b\u3089\u7c92\u5b50\u5f84\u3092\u6c42\u3081\u308b\u306b\u306f\u3001\u30b9\u30c8\u30fc\u30af\u30b9\u30a2\u30a4\u30f3\u30b7\u30e5\u30bf\u30a4\u30f3\u65b9\u7a0b\u5f0f\u304c\u4fbf\u5229\u3067\u3059\u3002<\/p>\n<figure itemscope itemtype=\"https:\/\/schema.org\/ImageObject\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/10\/DLS-equation-1@2x.png\" alt=\"DLS-equation 1@2x\" height=\"126\" width=\"400\" title=\"DLS-equation 1@2x\" onerror=\"this.style.display='none'\" loading=\"lazy\" \/>\n\t<\/figure>\n<figure itemscope itemtype=\"https:\/\/schema.org\/ImageObject\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/11\/DLS-light-scattering-1@2x.jpg\" alt=\"DLS-light-scattering 1@2x\" height=\"634\" width=\"696\" title=\"DLS-light-scattering 1@2x\" onerror=\"this.style.display='none'\" loading=\"lazy\" \/>\n\t<\/figure>\n\t<p>Figure 1. Light scattering intensity changes differently over time for different sized particles.<\/p>\n<figure itemscope itemtype=\"https:\/\/schema.org\/ImageObject\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/11\/DLS_correlation-1@2x.jpg\" alt=\"DLS_correlation 1@2x\" height=\"610\" width=\"612\" title=\"DLS_correlation 1@2x\" onerror=\"this.style.display='none'\" loading=\"lazy\" \/>\n\t<\/figure>\n\t<p>Figure 2. Correlation functions for proteins of two different sizes.<\/p>\n\t<p>\u3053\u306e\u5f0f\u306e\u4ed6\u306e\u5909\u6570\u3092\u5b8c\u6210\u3055\u305b\u308b\u305f\u3081\u306b\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u6e29\u5ea6\u3092\u6e2c\u5b9a\u3057\u3001\u7c98\u5ea6\u3092\u30e6\u30fc\u30b6\u30fc\u5b9a\u7fa9\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u8a08\u7b97\u3055\u308c\u305f\u76f4\u5f84\u306f\u6d41\u4f53\u529b\u5b66\u7684\u76f4\u5f84\u3067\u3059\u3002\u62e1\u6563\u4fc2\u6570\u6e2c\u5b9a\u306e\u6a19\u6e96\u504f\u5dee\u306f\u7c92\u5ea6\u5206\u5e03\u306e\u5e45\u306b\u63a5\u7d9a\u3055\u308c\u3001\u901a\u5e38\u306f\u591a\u5206\u6563\u6027\u6307\u6570\uff08PDI\uff09\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>DLS\u5206\u6790\u306e2\u756a\u76ee\u306e\u65b9\u6cd5\u306f\u3001DLS\u30c7\u30fc\u30bf\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u5229\u7528\u3057\u3066\u6e2c\u5b9a\u30c7\u30fc\u30bf\u3092\u518d\u73fe\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u7c92\u5ea6\u5206\u5e03\u3092\u628a\u63e1\u3057\u307e\u3059\uff08\u56f33\uff09\u3002\u3053\u308c\u3089\u306e\u5206\u6790\u65b9\u6cd5\u306f\u3044\u305a\u308c\u3082\u3001DLS\u306b\u3088\u308b\u7c92\u5b50\u5f84\u5206\u6790\u306e\u6700\u65b0\u306eISO\u898f\u683c<a href=\"https:\/\/www.iso.org\/standard\/65410.html\" target=\"_blank\">ISO 22412:2017<\/a>\u306b\u6e96\u62e0\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<figure itemscope itemtype=\"https:\/\/schema.org\/ImageObject\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/DLS-Combo_r3-1536x600-1@2x-1536x60011-1.jpg\" alt=\"DLS-Combo_r3-1536x600-1@2x-1536x60011\" height=\"600\" width=\"686\" title=\"DLS-Combo_r3-1536x600-1@2x-1536x60011\" onerror=\"this.style.display='none'\" loading=\"lazy\" \/>\n\t<\/figure>\n<figure itemscope itemtype=\"https:\/\/schema.org\/ImageObject\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/DLS-Combo_r3-1536x600-1@2x-1536x6002-2.jpg\" alt=\"DLS-Combo_r3-1536x600-1@2x-1536x6002\" height=\"600\" width=\"718\" title=\"DLS-Combo_r3-1536x600-1@2x-1536x6002\" onerror=\"this.style.display='none'\" loading=\"lazy\" \/>\n\t<\/figure>\n\t<p>\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u5206\u91ce\u3067\u306f\u3001\u52d5\u7684\u5149\u6563\u4e71\u306f\u30b5\u30f3\u30d7\u30eb\u306e\u30b5\u30a4\u30ba\u8a08\u6e2c\u3067\u304d\u308b\u3060\u3051\u3067\u306a\u304f\u3001\u30b5\u30f3\u30d7\u30eb\u304c\u51dd\u96c6\u3057\u3066\u3044\u308b\u304b\u3069\u3046\u304b\u8fc5\u901f\u306b\u30c1\u30a7\u30c3\u30af\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5927\u304d\u306a\u7c92\u5b50\u306f\u5149\u3092\u6fc0\u3057\u304f\u6563\u4e71\u3059\u308b\u305f\u3081\u3001DLS\u306f\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u3054\u304f\u307e\u308c\u306a\u51dd\u96c6\u4f53\u3082\u691c\u51fa\u3067\u304d\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u51dd\u96c6\u306b\u8b66\u9418\u3092\u9cf4\u3089\u3059\u3053\u3068\u3067\u3001\u3059\u3067\u306b\u30d4\u30fc\u30af\u3092\u904e\u304e\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u30c7\u30fc\u30bf\u306b\u983c\u3063\u305f\u308a\u3001\u6297\u4f53\u304c\u30d4\u30fc\u30af\u6642\u3068\u540c\u3058\u3088\u3046\u306b\u6a5f\u80fd\u3057\u306a\u304f\u306a\u3063\u305f\u7406\u7531\u3092\u8aac\u660e\u3057\u305f\u308a\u3059\u308b\u5fc5\u8981\u304c\u306a\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<h5>\n\t\t\tUnchained Labs \u52d5\u7684\u5149\u6563\u4e71\u88c5\u7f6e\u306e\u6a5f\u80fd\uff1a\t<\/h5>\n\t<ul>\n<li aria-level=\"1\">1\u30b5\u30f3\u30d7\u30eb\u3042\u305f\u308a1\u5206\u672a\u6e80\u3067\u3001\u6b63\u78ba\u3067\u518d\u73fe\u6027\u306e\u3042\u308b\u30b5\u30a4\u30b8\u30f3\u30b0\u30c7\u30fc\u30bf\u3092\u7372\u5f97<\/li>\n<li>\u5fae\u91cf\u30b5\u30f3\u30d7\u30eb\u91cf\u3068\u4f4e\u6fc3\u5ea6\u3068\u3044\u3046\u4fbf\u5229\u306a\u7d44\u307f\u5408\u308f\u305b\u3067\u30c7\u30fc\u30bf\u3092\u7372\u5f97<\/li>\n<li>\u30b5\u30f3\u30d7\u30eb\u5168\u4f53\u306e\u5e73\u5747\u7c92\u5b50\u5f84<\/li>\n<li>\u8907\u6570\u306e\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u305f\u5834\u5408\u306e\u7c92\u5ea6\u5206\u5e03<\/li>\n<li>\u5f93\u6765\u306eSEC\u5206\u6790\u3067\u306f\u56f0\u96e3\u306a\u30b5\u30a4\u30ba\u306e\u51dd\u96c6\u4f53\u304b\u3089\u5e0c\u5c11\u306a\u51dd\u96c6\u4f53\u307e\u3067\u3001\u9ad8\u611f\u5ea6\u3067\u691c\u51fa\u53ef\u80fd<\/li>\n<li>0.3\uff5e1000nm\u306e\u30b5\u30a4\u30ba\u6e2c\u5b9a<\/li>\n<li>\u30ea\u30be\u30c1\u30fc\u30e0\u3067\u306f0.1mg\/mL\u307e\u3067\u3001\u5927\u304d\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5834\u5408\u306f\u4f4e\u6fc3\u5ea6\u307e\u3067\u6e2c\u5b9a\u53ef\u80fd<\/li>\n<\/ul>\n<p>\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u554f\u984c\u3092\u89e3\u6c7a\u3059\u308b\u305f\u3081\u306b\u8a2d\u8a08\u3055\u308c\u305f2\u3064\u306e\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306eDLS\u88c5\u7f6e\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\t\t\t<h3>Stunner<\/h3>\t\t\t\n\t\t\t\t<p>Stunner\u306f\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/uv-vis-spectroscopy\/\">UV\/Vis<\/a>\u3068<a href=\"https:\/\/www.unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">\u56de\u8ee2\u89d2\u52d5\u7684\u5149\u6563\u4e71<\/a>\u6e2c\u5b9a\u3092\u540c\u3058\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u540c\u6642\u306b\u884c\u3048\u308b\u552f\u4e00\u306e\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u30c6\u30af\u30cb\u30c3\u30af\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u6fc3\u5ea6\u3001\u6d41\u4f53\u529b\u5b66\u7684\u30b5\u30a4\u30ba\u3001\u591a\u5206\u6563\u6027\u3001\u51dd\u96c6\u4f53\u306e\u691c\u51fa\u3092\u3001\u308f\u305a\u304b2 \u00b5L\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u4e00\u5ea6\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u6fc3\u5ea6\u6e2c\u5b9a\u306f2\uff05\u4ee5\u5185\u306e\u6b63\u78ba\u6027\u30681\uff05\u4ee5\u5185\u306e\u7cbe\u5ea6\u3067\u6b63\u78ba\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u307e\u305f\u3001UV\/Vis\u5206\u6790\u3068DLS\u5206\u6790\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001k<sub>D<\/sub>\u307e\u305f\u306fB<sub>22<\/sub>\u30c7\u30fc\u30bf\u3092\u7528\u3044\u3066\u30b5\u30f3\u30d7\u30eb\u306e\u30b3\u30ed\u30a4\u30c9\u5b89\u5b9a\u6027\u3092\u72ec\u81ea\u306b\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002Stunner\u3067\u306f\u3001\u4eee\u5b9a\u3059\u308b\u4ee3\u308f\u308a\u306b\u3001\u3059\u3079\u3066\u306e\u30c7\u30fc\u30bf\u30dd\u30a4\u30f3\u30c8\u3067\u6b63\u78ba\u306a\u6fc3\u5ea6\u3092\u8aad\u307f\u53d6\u308a\u3001\u305d\u308c\u3092RADLS\u30c7\u30fc\u30bf\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u6eb6\u6db2\u4e2d\u3067\u3069\u306e\u3088\u3046\u306b\u76f8\u4e92\u4f5c\u7528\u3057\u3066\u3044\u308b\u304b\u3092\u793a\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"\/stunner\/\" title=\"STUNNER\u95a2\u9023\u60c5\u5831\" target=\"_self\"   role=\"button\" aria-label=\"STUNNER\u95a2\u9023\u60c5\u5831\">\n\t\t\t\t\t\t\tSTUNNER\u95a2\u9023\u60c5\u5831\n\t\t<\/a>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Stunner-Front-cc_crop_downsize.png\" alt=\"Stunner Front cc_crop_downsize\" title=\"Stunner Front cc_crop_downsize\" itemprop=\"image\"\/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2025\/01\/Aunty-Front-rev04_740_Website-Version.png\" alt=\"Aunty-Front-rev04_740_Website Version\" title=\"Aunty-Front-rev04_740_Website Version\" itemprop=\"image\"\/>\n\t\t\t<h3>Aunty<\/h3>\t\t\t\n\t\t\t\t<p>Aunty\u306f\u3001\u86cd\u5149\u6e2c\u5b9a\u3001\u9759\u7684\u5149\u6563\u4e71\u3001\u52d5\u7684\u5149\u6563\u4e71\u306e\u6280\u8853\u309296\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u3067\u306e\u30b5\u30f3\u30d7\u30eb\u89e3\u6790\u306b\u7d44\u307f\u5408\u308f\u305b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b89\u5b9a\u6027\u306b\u95a2\u3059\u308b\u3059\u3079\u3066\u306e\u7591\u554f\u306b\u7b54\u3048\u308b\u7d50\u679c\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u9759\u7684\u5149\u6563\u4e71\u306f\u3001\u6bd4\u985e\u306e\u306a\u3044\u611f\u5ea6\u3067\u51dd\u96c6\u4f53\u304c\u5f62\u6210\u3055\u308c\u308b\u30bf\u30a4\u30df\u30f3\u30b0\u3092\u660e\u3089\u304b\u306b\u3057\u3001\u52d5\u7684\u5149\u6563\u4e71\u306f\u305d\u306e\u30b5\u30a4\u30ba\u30c7\u30fc\u30bf\u3092\u51fa\u3057\u307e\u3059\u3002Aunty\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b89\u5b9a\u6027\u306b\u95a2\u3059\u308b\u3042\u3089\u3086\u308b\u8cea\u554f\u306b\u3001\u9ad8\u901f\u304b\u3064\u512a\u308c\u305f\u30c7\u30fc\u30bf\u54c1\u8cea\u3067\u5bfe\u5fdc\u3059\u308b\u67d4\u8edf\u306a\u30c4\u30fc\u30eb\u3067\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5c55\u958b\u3057\u51dd\u96c6\u3059\u308b\u5168\u4f53\u306e\u904e\u7a0b\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u304c\u3069\u306e\u3088\u3046\u306b\u632f\u308b\u821e\u3063\u3066\u3044\u308b\u306e\u304b\u3092\u3001\u6642\u9593\u306e\u7d4c\u904e\u3068\u3068\u3082\u306b\u3001\u307e\u305f\u306f\u71b1\u52fe\u914d\u3092\u304b\u3051\u305f\u72b6\u614b\u3067\u6b63\u78ba\u306b\u628a\u63e1\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/aunty\/\" title=\"Aunty\u95a2\u9023\u60c5\u5831\" target=\"_self\"   role=\"button\" aria-label=\"Aunty\u95a2\u9023\u60c5\u5831\">\n\t\t\t\t\t\t\tAunty\u95a2\u9023\u60c5\u5831\n\t\t<\/a>\n<h2>\n\t\t\tFrequently Asked Questions\t<\/h2>\n\t\t\t\t\t\t\t\t\t\t<h3 tabindex=\"0\">What instrument is used to measure light scattering?<\/h3>\n\t\t\t\t\t<p>Two Unchained Labs instruments measure light scattering. Uncle uses both static and dynamic light scattering (SLS and DLS) to get you sample stability over time or in a temperature gradient. SLS monitors aggregation (as the average molar mass of the analyte), DLS keeps an eye on the hydrodynamic size.<\/p>\n<p>The second instrument that measures light scattering is Stunner &#8211; as rotational angle dynamic light scattering (RADLS): this evolution of single angle DLS is incredibly accurate and precise and keeps tabs on any aggregate present in your sample. Stunner also reports SLS at these angles and gives you the molar mass via the Raleigh ratio.<\/p>\n<p>\u00a0<\/p>\n\t\t\t\t\t\t\t\t\t\t<h3 tabindex=\"0\">How do you do dynamic light scattering?<\/h3>\n\t\t\t\t\t<p>In order to measure dynamic light scattering, you look at light scattered over a time interval. The molecules and particles in solution are excited by a laser which causes them to scatter light. As the analytes constantly move around (due to Brownian motion), the light scattered in a certain direction interferes constructively or destructively and the net intensity fluctuates over time &#8211; the larger the analyte, the slower the fluctuation.<\/p>\n<p>By analyzing the fluctuation through autocorrelation analysis, we can determine the translational diffusion coefficient of the particle which can then be converted to hydrodynamic size using the Stokes-Einstein equation.<\/p>\n<p>A second datapoint from a DLS experiment is the polydispersity of the sample, this is a measure of its homogeneity and a metric of the spread of sizes present within the sample.<\/p>\n\t\t\t\t\t\t\t\t\t\t<h3 tabindex=\"0\">What is the principle of measurement of a DLS instrument?<\/h3>\n\t\t\t\t\t<p>In a DLS experiment the fluctuation of light scattered at a discrete angle over time is measured by a photon counting module. A correlator coverts fluctuations over time to information about translational diffusion properties which can, with knowledge of temperature of measurement and viscosity of solution, be converted to hydrodynamic size.<\/p>\n<p>Depending on the type of analysis, a DLS experiment results in the z-average size and polydispersity or a distribution of sizes and their polydispersity.<\/p>\n\t\t\t\t\t\t\t\t\t\t<h3 tabindex=\"0\">What is the difference between DLS and SAXS?<\/h3>\n\t\t\t\t\t<p>Both dynamic light scattering (DLS) and small angle X-ray scattering (SAXS) measure scattering of electromagnetic radiation by an analyte. The incident beam excites electrons in the analyte and they emit the scattered light upon return to ground state.<\/p>\n<p>The X-rays in a SAXS measurement are more energetic and this experiment can yield size and information on the internal structure of the analyte.<\/p>\n<p>As DLS uses visible light to yield information about size and homogeneity, it requires a laser as the source of excitation and not an X-ray source.<\/p>\n\t\t\t\t\t\t\t\t\t\t<h3 tabindex=\"0\">What is the difference between static and dynamic light scattering?<\/h3>\n\t\t\t\t\t<p>Static light scattering (SLS) measures the total intensity of light scattered at a certain angle. This intensity, with knowledge of concentration and refractive index of the analyte, can be used to determine molar masses. Monitoring SLS over time or a temperature gradient lets you spot aggregation as it occurs.<\/p>\n<p>Dynamic light scattering (DLS) measures the fluctuations of light scattering intensity over time. This gives access to the translational diffusion of the analyte, which can be converted to hydrodynamic size and polydispersity.<\/p>\n<p>Though similar in name, SLS and DLS measure different properties of analytes and are orthogonal techniques.<\/p>\n<h3>\n\t\t\t\u3088\u308a\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u5834\u5408\u306f\u3053\u3061\u3089\t<\/h3>\n\t<p>\u52d5\u7684\u5149\u6563\u4e71\u88c5\u7f6e\u3092\u4f7f\u3048\u3070\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u8ab2\u984c\u3092\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u4f55\u304b\u3054\u4e0d\u660e\u306a\u70b9\u304c\u3054\u3056\u3044\u307e\u3057\u305f\u3089\u304a\u6c17\u8efd\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n<a href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\/\" target=\"_self\">\u304a\u554f\u3044\u5408\u308f\u305b<\/a>\n","protected":false},"excerpt":{"rendered":"<p>\u52d5\u7684\u5149\u6563\u4e71\u6cd5\uff08DLS\uff09\u3068\u306f\uff5c\u539f\u7406\u3068\u7c92\u5b50\u5f84\u30fb\u51dd\u96c6\u306e\u6e2c\u5b9a \u30bf\u30f3\u30d1\u30af\u8cea\u30fb\u305d\u306e\u4ed6\u306e\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u52d5\u7684\u5149\u6563\u4e71\u6e2c\u5b9a \u6280\u8853 \u95a2\u9023\u88fd\u54c1 &#8211; Stunner \u95a2\u9023\u88fd\u54c1 &#8211; Aunty FAQ \u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u7814\u7a76\u958b\u767a\u8005\u5411\u3051\u52d5\u7684\u5149\u6563\u4e71\u88c5\u7f6e \u52d5\u7684\u5149\u6563\u4e71\uff08DLS\uff09\u6cd5\u306f\u3001\u6eb6\u6db2\u4e2d\u306e\u5206\u5b50\u3084\u7c92\u5b50\u306b\u3088\u3063\u3066\u6563\u4e71\u3055\u308c\u308b\u30ec\u30fc\u30b6\u30fc\u5149\u5f37\u5ea6\u306e\u6025\u6fc0\u306a\u5909\u5316\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u7c92\u5b50\u5f84\u3068\u7c92\u5b50\u5f84\u5206\u5e03\u3092\u628a\u63e1\u3059\u308b\u6280\u8853\u3067\u3059\u3002DLS\u5206\u6790\u306f\u3001\u30da\u30d7\u30c1\u30c9\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30a6\u30a4\u30eb\u30b9\u3001VLP\u306a\u3069\u3001\u591a\u304f\u306e\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u30b5\u30a4\u30ba\u3092\u7d20\u65e9\u304f\u3001\u30e9\u30d9\u30eb\u30d5\u30ea\u30fc\u3067\u3001\u975e\u7834\u58ca\u7684\u306b\u628a\u63e1\u3059\u308b\u65b9\u6cd5\u3067\u3059\u300210nm\u306e\u6297\u4f53\u300130nm\u306eAAV\u3001\u307e\u305f\u306f\u30b5\u30d6\u30df\u30af\u30ed\u30f3\u30b5\u30a4\u30ba\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u30b5\u30f3\u30d7\u30eb\u306e\u5e73\u5747\u30b5\u30a4\u30ba\u3068\u51dd\u96c6\u72b6\u614b\u3092\u8aad\u307f\u53d6\u308b\u306b\u306f\u3001\u751f\u7269\u88fd\u5264\u7814\u7a76\u8005\u306b\u3068\u3063\u3066\u6700\u826f\u306e\u9078\u629e\u80a2\u3067\u3059\u3002\u307e\u305f\u3001\u56de\u8ee2\u89d2\u52d5\u7684\u5149\u6563\u4e71\uff08RADLS\uff09\u6cd5\u306f\u3001\u8907\u6570\u306e\u89d2\u5ea6\u304b\u3089\u30c7\u30fc\u30bf\u3092\u53ce\u96c6\u3059\u308b\u3053\u3068\u3067\u3001DLS\u3092\u57fa\u790e\u3068\u3059\u308b\u3055\u3089\u306b\u5f37\u529b\u306a\u6280\u8853\u3067\u3059\u3002 Unchained Labs\u306b\u306f\u3001\u554f\u984c\u89e3\u6c7a\u306e\u305f\u3081\u306e\u7a76\u6975\u306eDLS\u88c5\u7f6e\u3001Stunner\u3068Aunty\u304c\u3042\u308a\u307e\u3059\u3002Stunner\u3068Aunty\u306f\u3001\u3069\u3061\u3089\u3082\u5fae\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u7a3c\u50cd\u3067\u304d\u307e\u3059\u304c\u3001\u5404\u88c5\u7f6e\u306b\u305d\u308c\u305e\u308c\u306e\u7279\u9577\u304c\u3042\u308a\u307e\u3059\u3002\u6fc3\u5ea6\u3068\u30b5\u30a4\u30ba\u3092\u3059\u3070\u3084\u304f\u6e2c\u5b9a\u3057\u305f\u3051\u308c\u3070Stunner\u3092\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5b89\u5b9a\u6027\u306e\u5168\u4f53\u50cf\u3092\u628a\u63e1\u3057\u305f\u3051\u308c\u3070\u3001Aunty\u304c\u305d\u308c\u305e\u308c\u306e\u76ee\u7684\u306b\u9069\u3057\u305f\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002 \u52d5\u7684\u5149\u6563\u4e71\u6cd5\u306e\u539f\u7406 &nbsp; \u7c92\u5b50\u306e\u6eb6\u6db2\u306b\u30ec\u30fc\u30b6\u30fc\u5149\u3092\u7167\u5c04\u3059\u308b\u3068\u3001\u591a\u304f\u306e\u6563\u4e71\u5149\u3092\u691c\u51fa\u3067\u304d\u307e\u3059\u3002\u30ec\u30fc\u30b6\u30fc\u306e\u6ce2\u9577\u304c\u7c92\u5b50\u30b5\u30a4\u30ba\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u5927\u304d\u3044\u3068\u3001\u3069\u306e\u65b9\u5411\u306b\u3082\u540c\u3058\u91cf\u306e\u5149\u304c\u62e1\u6563\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001DLS\u6a5f\u5668\u306b\u306f660nm\u30ec\u30fc\u30b6\u30fc\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002 DLS\u306f\u3001\u6563\u4e71\u5f37\u5ea6\u304c\u6642\u9593\u306e\u7d4c\u904e\u3068\u5171\u306b\u3069\u308c\u3060\u3051\u6025\u901f\u306b\u5909\u5316\u3059\u308b\u304b\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u6eb6\u6db2\u4e2d\u306e\u7c92\u5b50\u306e\u30b5\u30a4\u30ba\u306b\u3064\u3044\u3066\u591a\u304f\u306e\u3053\u3068\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u56f31\uff09\u3002\u5c0f\u3055\u306a\u7c92\u5b50\u306f\u7d20\u65e9\u304f\u52d5\u304d\u56de\u308b\u306e\u3067\u3001\u5149\u306e\u5f37\u5ea6\u306f\u7d20\u65e9\u304f\u5909\u5316\u3057\u307e\u3059\u3002\u5927\u304d\u306a\u7c92\u5b50\u306f\u52d5\u304d\u56de\u308b\u306e\u304c\u9045\u3044\u306e\u3067\u3001\u305d\u306e\u9006\u3067\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u5149\u5f37\u5ea6\u306e\u5909\u5316\u304c\u901f\u3044\u304b\u9045\u3044\u304b\u3092\u5206\u6790\u3059\u308b\u306e\u304cDLS\u5206\u6790\u3067\u3059\u3002 DLS\u306e\u5149\u6563\u4e71\u30c7\u30fc\u30bf\u306e\u5206\u6790\u306b\u3064\u3044\u3066\u8003\u3048\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u3042\u308b\u6642\u70b9\u3092\u9078\u629e\u3057\u30011\u30de\u30a4\u30af\u30ed\u79d2\u65e9\u9001\u308a\u3057\u307e\u3059\u3002\u307b\u3068\u3093\u3069\u306e\u7c92\u5b50\u306f\u307e\u3060\u52d5\u304d\u56de\u3063\u3066\u3044\u306a\u3044\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u306e\u3067\u3001\u5149\u6563\u4e71\u306f\u5909\u5316\u3057\u3066\u304a\u3089\u305a\u3001\u305d\u306e\u30bc\u30ed\u6642\u70b9\u306e\u30c7\u30fc\u30bf\u30681\u30de\u30a4\u30af\u30ed\u79d2\u5f8c\u306e\u30c7\u30fc\u30bf\u306f\u307b\u307c\u540c\u3058\u3067\u3059\u3002\u3064\u307e\u308a\u3001\u9ad8\u3044\u76f8\u95a2\u95a2\u4fc2\u304c\u3042\u308b\u306e\u3067\u3059\u3002 \u6b21\u306f\uff11\u30de\u30a4\u30af\u30ed\u79d2\u306e\u4ee3\u308f\u308a\u306b\u30011\u79d2\u5f8c\u306e\u30c7\u30fc\u30bf\u3092\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u307b\u3068\u3093\u3069\u306e\u7c92\u5b50\u306f\u307e\u3063\u305f\u304f\u9055\u3046\u5834\u6240\u306b\u3042\u308a\u3001\u958b\u59cb\u70b9\u30681\u79d2\u5f8c\u306e\u9593\u306e\u30c7\u30fc\u30bf\u306e\u76f8\u95a2\u306f\u30bc\u30ed\u306b\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u76f8\u95a2\u5024\u3092\u3055\u307e\u3056\u307e\u306a\u6642\u9593\u306e\u7d4c\u904e\u306b\u3064\u3044\u3066\u30b0\u30e9\u30d5\u306b\u3059\u308b\u3068\u3001\u76f8\u95a2\u95a2\u6570\u304c\u5f97\u3089\u308c\u307e\u3059\uff08\u56f32\uff09\u3002\u305d\u3057\u3066\u3001\u76f8\u95a2\u304c\u9ad8\u3044\u72b6\u614b\u304b\u3089\u76f8\u95a2\u304c\u30bc\u30ed\u306b\u306a\u308b\u307e\u3067\u306e\u901f\u3055\u304b\u3089\u3001\u7c92\u5b50\u306e\u5e73\u5747\u30b5\u30a4\u30ba\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u52d5\u7684\u5149\u6563\u4e71\u304c\u5149\u5b50\u76f8\u95a2\u5206\u5149\u6cd5\uff08PCS\uff09\u3068\u547c\u3070\u308c\u308b\u7406\u7531\u3067\u3082\u3042\u308a\u307e\u3059\u3002 \u76f8\u95a2\u95a2\u6570\u304b\u3089\u3001\u672c\u5f53\u306b\u5f97\u305f\u3044\u30c7\u30fc\u30bf\u306b\u305f\u3069\u308a\u7740\u304f\u306b\u306f\u30012\u3064\u306e\u5206\u6790\u65b9\u6cd5\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002\u4e00\u3064\u76ee\u306e\u65b9\u6cd5\u306f\u3001\u76f8\u95a2\u95a2\u6570\u306b\u300c\u30d9\u30b9\u30c8\u30d5\u30a3\u30c3\u30c8\u300d\u3092\u9069\u7528\u3057\u3001\u305d\u306e\u30d5\u30a3\u30c3\u30c8\u306e\u5f62\u72b6\u304b\u3089\u62e1\u6563\u4fc2\u6570\u3001\u5e73\u5747\u7c92\u5b50\u5f84\u3001\u7c92\u5b50\u5f84\u5206\u5e03\u3092\u6c42\u3081\u308b\u306e\u3067\u3059\u3002\u62e1\u6563\u4fc2\u6570\u304b\u3089\u7c92\u5b50\u5f84\u3092\u6c42\u3081\u308b\u306b\u306f\u3001\u30b9\u30c8\u30fc\u30af\u30b9\u30a2\u30a4\u30f3\u30b7\u30e5\u30bf\u30a4\u30f3\u65b9\u7a0b\u5f0f\u304c\u4fbf\u5229\u3067\u3059\u3002 Figure 1. Light scattering intensity changes differently over time for different sized particles. Figure 2. Correlation functions for proteins of two different sizes. \u3053\u306e\u5f0f\u306e\u4ed6\u306e\u5909\u6570\u3092\u5b8c\u6210\u3055\u305b\u308b\u305f\u3081\u306b\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u6e29\u5ea6\u3092\u6e2c\u5b9a\u3057\u3001\u7c98\u5ea6\u3092\u30e6\u30fc\u30b6\u30fc\u5b9a\u7fa9\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u8a08\u7b97\u3055\u308c\u305f\u76f4\u5f84\u306f\u6d41\u4f53\u529b\u5b66\u7684\u76f4\u5f84\u3067\u3059\u3002\u62e1\u6563\u4fc2\u6570\u6e2c\u5b9a\u306e\u6a19\u6e96\u504f\u5dee\u306f\u7c92\u5ea6\u5206\u5e03\u306e\u5e45\u306b\u63a5\u7d9a\u3055\u308c\u3001\u901a\u5e38\u306f\u591a\u5206\u6563\u6027\u6307\u6570\uff08PDI\uff09\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002 DLS\u5206\u6790\u306e2\u756a\u76ee\u306e\u65b9\u6cd5\u306f\u3001DLS\u30c7\u30fc\u30bf\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u3092\u5229\u7528\u3057\u3066\u6e2c\u5b9a\u30c7\u30fc\u30bf\u3092\u518d\u73fe\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u7c92\u5ea6\u5206\u5e03\u3092\u628a\u63e1\u3057\u307e\u3059\uff08\u56f33\uff09\u3002\u3053\u308c\u3089\u306e\u5206\u6790\u65b9\u6cd5\u306f\u3044\u305a\u308c\u3082\u3001DLS\u306b\u3088\u308b\u7c92\u5b50\u5f84\u5206\u6790\u306e\u6700\u65b0\u306eISO\u898f\u683cISO 22412:2017\u306b\u6e96\u62e0\u3057\u3066\u3044\u307e\u3059\u3002 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