{"id":4837,"date":"2021-12-07T12:47:20","date_gmt":"2021-12-07T12:47:20","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=4837"},"modified":"2024-05-17T04:50:26","modified_gmt":"2024-05-17T04:50:26","slug":"titer-empty-full-ratio","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/titer-empty-full-ratio\/","title":{"rendered":"AAV titer &#038; empty\/full ratio"},"content":{"rendered":"\n<h1>\n\t\tAAV \u529b\u4fa1 &#038; Empty\/Full\u6bd4\u7387\n\t<\/h1>\n\t<ul>\n<li><a href=\"#problem\">\u8ab2\u984c<\/a><\/li>\n<li><a href=\"#solution\">\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/a><\/li>\n<li><a href=\"#proof\">\u5b9f\u8a3c\u30c7\u30fc\u30bf<\/a><\/li>\n<li><a href=\"#stunner\">\u95a2\u9023\u88fd\u54c1<strong> &#8211; Stunner<\/strong><\/a><\/li>\n<\/ul>\n<h4>\n\t\t\u8ab2\u984c\n\t<\/h4>\n\t<p>\u30a2\u30c7\u30ce\u968f\u4f34\u30a6\u30a4\u30eb\u30b9(AAV)\u306e\u529b\u4fa1\u3084AAV\u306eempty\/full\u6bd4\u7387\u3092\u8fc5\u901f\u304b\u3064\u52b9\u7387\u7684\u306b\u5b9a\u91cf\u3059\u308b\u3053\u3068\u306f\u3001\u958b\u767a\u3084\u88fd\u9020\u306b\u304a\u3051\u308b\u5927\u304d\u306a\u8ab2\u984c\u3067\u3059\u3002\u591a\u304f\u306e\u5834\u5408\u3053\u308c\u3089\u306e\u5b9a\u91cf\u30c7\u30fc\u30bf\u306b\u57fa\u3065\u3044\u3066\u610f\u601d\u6c7a\u5b9a\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u3001\u305d\u306e\u56de\u7b54\u306f\u8fc5\u901f\u304b\u3064\u7c21\u5358\u3067\u3001\u6700\u5c0f\u9650\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3067\u3042\u308b\u3053\u3068\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u3002<\/p>\n<p>AUC\u6cd5\u3001TEM\u6cd5\u3001HPLC\u6cd5\u3001ELISA\u6cd5\u3001PCR\u6cd5\u306a\u3069\u306f\u3001\u305d\u308c\u305e\u308c\u30b5\u30f3\u30d7\u30eb\u306b\u95a2\u3059\u308b\u60c5\u5831\u306e\u4e00\u90e8\u3092\u63d0\u4f9b\u3057\u307e\u3059\u304c\u3001\u3044\u305a\u308c\u3082\u8fc5\u901f\u3001\u7c21\u4fbf\u3001\u5c11\u91cf\u30b5\u30f3\u30d7\u30eb\u3068\u3044\u3046\u6761\u4ef6\u3067\u7d50\u679c\u3092\u5f97\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002AUC\u306f\u30b4\u30fc\u30eb\u30c9\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u3082\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u6e2c\u5b9a\u6cd5\u3067\u3059\u304c\u3001\u81a8\u5927\u306a\u6642\u9593\u3068\u30b5\u30f3\u30d7\u30eb\u3001\u305d\u3057\u3066\u7279\u6b8a\u306a\u88c5\u7f6e\u304c\u5fc5\u8981\u3067\u3059\u3002\u307e\u305f\u3001ELISA\u3068PCR\u306f\u5805\u5b9f\u306a\u624b\u6cd5\u3067\u3059\u304c\u3001\u4f55\u6642\u9593\u3082\u304b\u304b\u308a\u3001\u305d\u308c\u305e\u308c\u306eAAV\u30d9\u30af\u30bf\u30fc\u7528\u306b\u958b\u767a\u3068\u6700\u9069\u5316\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<h4>\n\t\t\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\n\t<\/h4>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/stunner\">Stunner<\/a>\u306f\u3001AAV\u529b\u4fa1\u3001AAV Empty\/Full\u6bd4\u7387\u3001\u51dd\u96c6\u3092\u3001\u308f\u305a\u304b2 \u00b5L\u3092\u4f7f\u7528\u3057\u3066\u7d041\u5206\u3067\u5b8c\u5168\u306b\u8aad\u307f\u53d6\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002Stunner\u306f<a href=\"https:\/\/unchainedlabs.com\/jp\/uv-vis-spectroscopy\">UV\/Vis\u5206\u5149\u6cd5<\/a>\u3068<a href=\"https:\/\/unchainedlabs.com\/jp\/static-light-scattering\">\u9759\u7684<\/a>\u30fb<a href=\"https:\/\/unchainedlabs.com\/jp\/dynamic-light-scattering\">\u52d5\u7684\u5149\u6563\u4e71<\/a>\uff08SLS &amp; DLS\uff09\u3092\u7d44\u307f\u5408\u308f\u305b\u3001\u308f\u305a\u304b1\u6642\u9593\u306710<sup>12<\/sup> vg\/mL\u307e\u3067\u306e\u529b\u4fa1\u3092\u6e2c\u5b9a\u3057\u300196\u30b5\u30f3\u30d7\u30eb\u306e\u30b5\u30a4\u30ba\u3068\u51dd\u96c6\u3092\u30c1\u30a7\u30c3\u30af\u3057\u307e\u3059\u3002Stunner\u306f\u3001\u3042\u3089\u3086\u308bAAV\u8840\u6e05\u578b\u307e\u305f\u306f\u30a4\u30f3\u30b5\u30fc\u30c8\u306b\u5bfe\u3057\u3066\u3001\u8272\u7d20\u30d5\u30ea\u30fc\u3001\u30e9\u30d9\u30eb\u30d5\u30ea\u30fc\u3001\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u30d5\u30ea\u30fc\u3001\u624b\u9593\u3044\u3089\u305a\u306e\u5b9a\u91cf\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/p>\n<h4>\n\t\t\u5b9f\u8a3c\u30c7\u30fc\u30bf\n\t<\/h4>\n\t<p>DLS\u304a\u3088\u3073SLS\u3068UV\/Vis\u3092\u7d44\u307f\u5408\u308f\u305b\u305fStunner\u72ec\u81ea\u306e\u6a5f\u80fd\u306b\u3088\u308a\u3001AAV\u306e\u7dcf\u529b\u4fa1\u3001\u30d5\u30eb\u30ad\u30e3\u30d7\u30b7\u30c9\u529b\u4fa1\u3001\u30b5\u30f3\u30d7\u30eb\u306eEmpty\/Full\u6bd4\u7387\u3092\u7b97\u51fa\u3059\u308b\u306e\u306b\u5341\u5206\u306a\u60c5\u5831\u304c\u5f97\u3089\u308c\u307e\u3059\u3002Stunner\u306eDLS\u306f\u30b5\u30f3\u30d7\u30eb\u3092\u8aad\u307f\u53d6\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u7c92\u5b50\u306e\u30b5\u30a4\u30ba\u3068\u30b5\u30a4\u30ba\u5206\u5e03\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002DLS\u306e\u5f37\u5ea6\u5206\u5e03\u306f\u3001\u6563\u4e71\u5149\u306e\u7dcf\u5f37\u5ea6\u3092AAV\u30ad\u30e3\u30d7\u30b7\u30c9\u306b\u3088\u308b\u6563\u4e71\u5149\u3060\u3051\u306b\u7d5e\u308a\u8fbc\u3080\u306e\u306b\u5f79\u7acb\u3061\u307e\u3059\u3002SLS\u306e\u5f37\u5ea6\u306f\u7c92\u5b50\u6fc3\u5ea6\u306b\u6b63\u6bd4\u4f8b\u3057\u307e\u3059\u304c\u3001\u30d5\u30eb\u306e\u30ad\u30e3\u30d7\u30b7\u30c9\u3068\u7a7a\u306e\u30ad\u30e3\u30d7\u30b7\u30c9\u3067\u306f\u6563\u4e71\u3059\u308b\u5149\u306e\u91cf\u304c\u7570\u306a\u308b\u305f\u3081\u3001\u6700\u3082\u6b63\u78ba\u306aAAV\u529b\u4fa1\u3092\u6e2c\u5b9a\u3059\u308b\u306b\u306fDLS\u3068SLS\u3060\u3051\u3067\u306a\u304f\u3001UV\/Vis\u30c7\u30fc\u30bf\u3082\u5fc5\u8981\u3067\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/DLS_SLS_UV_Vis-graphs-1.png\" alt=\"DLS_SLS_UV_Vis-graphs-1\" itemprop=\"image\" height=\"409\" width=\"435\" title=\"DLS_SLS_UV_Vis-graphs-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/DLS_SLS_UV_Vis-graphs-2.png\" alt=\"DLS_SLS_UV_Vis-graphs-2\" itemprop=\"image\" height=\"401\" width=\"415\" title=\"DLS_SLS_UV_Vis-graphs-2\" onerror=\"this.style.display='none'\"  \/>\n\t<p>UV\/Vis\u5206\u5149\u6cd5\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u5b58\u5728\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3068DNA\u306e\u7dcf\u91cf\u3092\u6e2c\u5b9a\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3068DNA\u306e\u6bd4\u7387\u304b\u3089\u7cbe\u88fdAAV\u306eEmpty\/Full\u6bd4\u7387\u3092\u8aad\u307f\u53d6\u308a\u307e\u3059\u3002Stunner\u304cUV\/Vis\u3068DLS\u304a\u3088\u3073SLS\u306e\u7d50\u679c\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3068\u3001\u30ad\u30e3\u30d7\u30b7\u30c9\u304b\u3089\u306e\u6563\u4e71\u5149\u3092\u6e2c\u5b9a\u3059\u308b\u306e\u306b\u5341\u5206\u306a\u60c5\u5831\u304c\u5f97\u3089\u308c\u3001\u305d\u308c\u3089\u306e\u30ad\u30e3\u30d7\u30b7\u30c9\u306e\u3046\u3061\u4f55\u30d1\u30fc\u30bb\u30f3\u30c8\u304c\u30d5\u30eb\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u304b\u3092\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001AAV\u306e\u7dcf\u529b\u4fa1\u3001\u30d5\u30eb\u30ad\u30e3\u30d7\u30b7\u30c9\u529b\u4fa1\u3001Empty\/Full\u6bd4\u7387\u3092\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u904e\u5270\u306aDNA\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u304cUV\/Vis\u3067\u691c\u51fa\u3055\u308c\u305f\u5834\u5408\u3001\u305d\u308c\u306f\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u904a\u96e2\u51dd\u96c6DNA\u3084\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u547c\u3070\u308c\u307e\u3059\u3002\u3000<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/capsid-titer_empty_full-graphs-1.jpg\" alt=\"capsid-titer_empty_full-graphs-1\" itemprop=\"image\" height=\"534\" width=\"454\" title=\"capsid-titer_empty_full-graphs-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/capsid-titer_empty_full-graphs-2.jpg\" alt=\"capsid-titer_empty_full-graphs-2\" itemprop=\"image\" height=\"519\" width=\"447\" title=\"capsid-titer_empty_full-graphs-2\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Stunner-Front-cc_crop_downsize.png\" alt=\"Stunner Front cc_crop_downsize\" title=\"Stunner Front cc_crop_downsize\" itemprop=\"image\"\/>\n\t\t\t<h3>Stunner<\/h3>\t\t\t\n\t\t\t\t<p>Stunner\u306f\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/uv-vis-spectroscopy\">UV\/Vis<\/a>\u6fc3\u5ea6\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/dynamic-light-scattering\">\u52d5\u7684\u5149\u6563\u4e71\uff08DLS\uff09<\/a>\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/static-light-scattering\">\u9759\u7684\u5149\u6563\u4e71\uff08SLS\uff09<\/a>\u30c7\u30fc\u30bf\u3092\u540c\u30582 \u03bcL\u306e\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u53d6\u5f97\u3067\u304d\u308b\u552f\u4e00\u306e\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002AAV\u3092\u6398\u308a\u4e0b\u3052\u3066\u3001\u30ad\u30e3\u30d7\u30b7\u30c9\u306e\u7dcf\u529b\u4fa1\u3084Empty\/Full\u6bd4\u7387\u3092\u53d6\u5f97\u3057\u305f\u308a\u3001\u307e\u305fAAV\u4ee5\u5916\u3067\u3082\u3001\u4f8b\u3048\u3070\u69d8\u3005\u306a\u30ca\u30ce\u7c92\u5b50\u306e\u30da\u30a4\u30ed\u30fc\u30c9\u6fc3\u5ea6\u3084\u30b5\u30a4\u30ba\u30c7\u30fc\u30bf\u3092\u4e00\u5ea6\u306b\u53ce\u96c6\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u6fc3\u5ea6\u3001\u6d41\u4f53\u529b\u5b66\u7684\u30b5\u30a4\u30ba\u3001\u591a\u5206\u6563\u6027\u3001\u51dd\u96c6\u4f53\u306e\u691c\u51fa\u3092\u4e00\u767a\u3067\u30ea\u30b9\u30c8\u304b\u3089\u9664\u5916\u3059\u308b\u3053\u3068\u3067\u3001\u304a\u6301\u3061\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u54c1\u8cea\u3092\u6b63\u78ba\u306b\u628a\u63e1\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001Stunner\u3092\u4f7f\u3048\u3070\u3001AAV\u3001\u30ca\u30ce\u7c92\u5b50\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u54c1\u8cea\u3092\u3001\u77ac\u6642\u306b\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/stunner\" target=\"_self\" role=\"button\" aria-label=\"STUNNER\u3092\u77e5\u308b\" rel=\"noopener\">\n\t\t\t\t\t\t\tSTUNNER\u3092\u77e5\u308b\n\t\t<\/a>\n<h2>\n\t\t\u3082\u3063\u3068\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\uff1f\n\t<\/h2>\n\t<p>Stunner\u304c\u3069\u306e\u3088\u3046\u306b\u8272\u7d20\u30d5\u30ea\u30fc\u3001\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u30d5\u30ea\u30fc\u3001\u624b\u9593\u3044\u3089\u305a\u3067AAV\u529b\u4fa1\u304a\u3088\u3073Empty\/Full\u6bd4\u7387\u3092\u63d0\u4f9b\u3059\u308b\u304b\u3001\u3055\u3089\u306b\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u3067\u3059\u304b\uff1f<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u304a\u554f\u3044\u5408\u308f\u305b\n\t\t\t\t\t<\/a>\n<h2>\n\t\t\u95a2\u9023\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\n\t<\/h2>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/capsid-stability\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u30ad\u30e3\u30d7\u30b7\u30c9\u306e\u5b89\u5b9a\u6027\n\t\t\t\t\t<\/a>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/reformulation-concentration\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u518d\u88fd\u5264\u5316 &#038; \u6fc3\u7e2e\n\t\t\t\t\t<\/a>\n\n","protected":false},"excerpt":{"rendered":"<p>AAV \u529b\u4fa1 &#038; Empty\/Full\u6bd4\u7387 \u8ab2\u984c \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 \u5b9f\u8a3c\u30c7\u30fc\u30bf \u95a2\u9023\u88fd\u54c1 &#8211; Stunner \u8ab2\u984c \u30a2\u30c7\u30ce\u968f\u4f34\u30a6\u30a4\u30eb\u30b9(AAV)\u306e\u529b\u4fa1\u3084AAV\u306eempty\/full\u6bd4\u7387\u3092\u8fc5\u901f\u304b\u3064\u52b9\u7387\u7684\u306b\u5b9a\u91cf\u3059\u308b\u3053\u3068\u306f\u3001\u958b\u767a\u3084\u88fd\u9020\u306b\u304a\u3051\u308b\u5927\u304d\u306a\u8ab2\u984c\u3067\u3059\u3002\u591a\u304f\u306e\u5834\u5408\u3053\u308c\u3089\u306e\u5b9a\u91cf\u30c7\u30fc\u30bf\u306b\u57fa\u3065\u3044\u3066\u610f\u601d\u6c7a\u5b9a\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u3001\u305d\u306e\u56de\u7b54\u306f\u8fc5\u901f\u304b\u3064\u7c21\u5358\u3067\u3001\u6700\u5c0f\u9650\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3067\u3042\u308b\u3053\u3068\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u3002 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