{"id":4970,"date":"2021-12-08T05:07:45","date_gmt":"2021-12-08T05:07:45","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=4970"},"modified":"2024-05-17T04:52:14","modified_gmt":"2024-05-17T04:52:14","slug":"reformulation-concentration","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/reformulation-concentration\/","title":{"rendered":"AAV concentration &#038; reformulation"},"content":{"rendered":"\n<h1>\n\t\tAAV \u306e\u6fc3\u7e2e\u3068\u30ea\u30d5\u30a9\u30fc\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\n\t<\/h1>\n\t<ul>\n<li><a href=\"#problem\">\u8ab2\u984c<\/a><\/li>\n<li><a href=\"#solution\">\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/a><\/li>\n<li><a href=\"#proof\">\u5b9f\u8a3c\u30c7\u30fc\u30bf<\/a><\/li>\n<li><a href=\"#unagi\">\u95a2\u9023\u88fd\u54c1 <strong>&#8211; Unagi<\/strong><\/a><\/li>\n<li><a href=\"#big_tuna\">\u95a2\u9023\u88fd\u54c1 <strong>&#8211; Big Tuna<\/strong><\/a><\/li>\n<\/ul>\n<h4>\n\t\t\u8ab2\u984c\n\t<\/h4>\n\t<p>AAV\u306f\u5927\u62b5\u306e\u5834\u5408\u4f4e\u6fc3\u5ea6\u3067\u751f\u7523\u3055\u308c\u308b\u305f\u3081\u3001\u305d\u306e\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3068\u3068\u6fc3\u7e2e\u304c\u5fc5\u8981\u3067\u3059\u3002\u9060\u5fc3\u6ffe\u904e\u88c5\u7f6e\u30841\u56de1\u56de\u306e\u30d5\u30ed\u30fc\u6ffe\u904e\u88c5\u7f6e\u3067\u3053\u306e\u30d7\u30ed\u30bb\u30b9\u306b\u53d6\u308a\u7d44\u3080\u306e\u306f\u3001\u7279\u306b\u30b5\u30f3\u30d7\u30eb\u6570\u3084\u91cf\u304c\u591a\u3044\u5834\u5408\u3001\u624b\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u3002AAV\u306e\u3088\u3046\u306a\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5b8c\u5168\u6027\u3092\u640d\u306a\u308f\u305a\u3001\u304b\u3064\u9ad8\u3044\u56de\u53ce\u7387\u3092\u6301\u3064\u8fc5\u901f\u306a\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3068\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n<h4>\n\t\t\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\n\t<\/h4>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\">Unagi<\/a>\u306f\u3001AAV\u30b5\u30f3\u30d7\u30eb\u306e\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3068\u6fc3\u7e2e\u306b\u5fc5\u8981\u306a\u3001\u624b\u9593\u306e\u304b\u304b\u308b\u3059\u3079\u3066\u306e\u30b9\u30c6\u30c3\u30d7\u3092\uff11\u3064\u306b\u307e\u3068\u3081\u305f\u521d\u306e\u30d9\u30f3\u30c1\u30c8\u30c3\u30d7\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002Unagi\u306f<a href=\"https:\/\/unchainedlabs.com\/jp\/ultrafiltration-diafiltration-uf-df\">\u9650\u5916\u308d\u904e\/\u30c0\u30a4\u30a2\u30d5\u30a3\u30eb\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\uff08UF\/DF\uff09<\/a>\u3092\u4f7f\u7528\u3057\u3066\u30010.5\uff5e8mL\u306e\u958b\u59cb\u5bb9\u91cf\u304b\u30891\uff5e8\u30b5\u30f3\u30d7\u30eb\u307e\u3067\u4ea4\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Unagi\u306f\u5b9f\u884c\u4e2d\u306b\u30b5\u30f3\u30d7\u30eb\u306e\u5bb9\u91cf\u3092\u6ce8\u610f\u6df1\u304f\u30e2\u30cb\u30bf\u30fc\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u308d\u904e\u3057\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u3082\u3069\u3059\u3079\u304d\u5bb9\u91cf\u3092\u628a\u63e1\u3057\u3066\u3044\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u304c\u76ee\u6a19\u306e\u4ea4\u63db\u7387\u307e\u305f\u306fAAV\u6fc3\u5ea6\u306b\u9054\u3059\u308b\u307e\u3067\u3001\u3053\u308c\u3092\u7e70\u308a\u8fd4\u3057\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/03\/Take-it-down.png\" alt=\"Take it down\" itemprop=\"image\" height=\"575\" width=\"1000\" title=\"Take it down\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\">Unagi <\/a>\u306fUna\u3068\u547c\u3070\u308c\u308b\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u5404Una\u306f0.5\uff5e8mL\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u4fdd\u6301\u3057\u3001Una\u306f1\u30e9\u30f3\u3042\u305f\u308a1\uff5e8\u30b5\u30f3\u30d7\u30eb\u3092\u53d6\u308a\u6271\u3044\u307e\u3059\u3002\u5206\u5b50\u91cf10\u300130\u3001100kDa\u306e\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u306f\u5438\u53ce\u3055\u308c\u308b\u3053\u3068\u306a\u304f\u300196\uff05\u4ee5\u4e0a\u306e\u56de\u53ce\u7387\u3067\u6db2\u4f53\u3092\u901a\u904e\u3057\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/03\/Served-up-ala-carte.png\" alt=\"Served up a&#039;la carte\" itemprop=\"image\" height=\"473\" width=\"900\" title=\"Served up a&#039;la carte\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/big-tuna\/\">Big Tuna<\/a>\u306f\u3001\u3088\u308a\u5747\u4e00\u306a\u30b5\u30f3\u30d7\u30eb\u30cf\u30f3\u30c9\u30ea\u30f3\u30b0\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u3068\u3001\u30de\u30cb\u30e5\u30a2\u30eb\u6cd5\u3067\u306f\u5f97\u3089\u308c\u306a\u3044\u9ad8\u5ea6\u306a\u30d7\u30ed\u30bb\u30b9\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u63d0\u4f9b\u3059\u308b\u5b8c\u5168\u81ea\u52d5\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002Big Tuna\u306f\u3001\u6539\u826f\u578b<a href=\"https:\/\/unchainedlabs.com\/jp\/ultrafiltration-diafiltration-uf-df\">\u9650\u5916\u308d\u904e\/\u900f\u6790\u308d\u904e\uff08UF\/DF\uff09<\/a>\u6cd5\u3092\u7528\u3044\u3066\u3001AAV\u3084\u305d\u306e\u4ed6\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3092\u4ea4\u63db\u30fb\u6fc3\u7e2e\u3057\u307e\u3059\u3002Big Tuna\u306f\u3001\u904b\u8ee2\u4e2d\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u30e2\u30cb\u30bf\u30fc\u3057\u3001\u308d\u904e\u3055\u308c\u305f\u91cf\u3092\u623b\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u6bce\u56de\u9069\u5207\u306a\u4ea4\u63db\u7387\u3068\u6fc3\u5ea6\u306b\u3057\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/large-get-concentrated-45.png\" alt=\"large-get-concentrated-45\" itemprop=\"image\" height=\"289\" width=\"1000\" title=\"large-get-concentrated-45\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/big-tuna\/\">Big Tuna<\/a>\u3067\u306f\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/45_Unfilter_spec_sheet.pdf\" target=\"_blank\" rel=\"noopener\">Unfilter<\/a>\u3068\u547c\u3070\u308c\u308b\u30d7\u30ec\u30fc\u30c8\u30d9\u30fc\u30b9\u306e\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u3059\u3079\u3066\u306eUnfilter\u306f\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3092\u4f7f\u7528\u3057\u3066\u304a\u308a\u3001\u975e\u7279\u7570\u7684\u7d50\u5408\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u6700\u9069\u3067\u3059\u300224\u30a6\u30a7\u30eb\uff080.45 &#8211; 8 mL\uff09\u306896\u30a6\u30a7\u30eb\uff08100 &#8211; 450 \u00b5L\uff09\u306e2\u3064\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\u3002Unfilter 96\u306f\u3001\u5206\u5b50\u91cf\u30ab\u30c3\u30c8\u30aa\u30d5\u304c3\u300110\u300130\u3001100 kDa\u3002Unfilter 24\u306e\u5206\u5b50\u91cf\u306f10\u300130\u3001100 kDa\u3067\u3059\u3002\u5206\u5b50\u91cf\u306e\u9ad8\u3044\u30ab\u30c3\u30c8\u30aa\u30d5\u5024\u306f\u3001\u5927\u304d\u306a\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u6700\u9069\u3067\u3059\u3002Unfilter 24 \u306f\u6700\u5927 48 mL \u3092 8 mL \u307e\u3067\u6fc3\u7e2e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/2plates.png\" alt=\"2plates\" itemprop=\"image\" height=\"259\" width=\"900\" title=\"2plates\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\t\u5b9f\u8a3c\u30c7\u30fc\u30bf\n\t<\/h4>\n<h5>\n\t\tAAV\u30b5\u30f3\u30d7\u30eb\u306e\u6700\u9069\u5316\u3068\u5b89\u5b9a\u6027\u8a55\u4fa1\n\t<\/h5>\n\t<p>AAV\u306e\u5b89\u5b9a\u6027\u306f\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u306epH\u3001\u5869\u3001\u30a4\u30aa\u30f3\u5f37\u5ea6\u306e\u5909\u5316\u306b\u3088\u3063\u3066\u5f71\u97ff\u3092\u53d7\u3051\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\">Unagi<\/a>\u307e\u305f\u306f<a href=\"https:\/\/unchainedlabs.com\/jp\/big-tuna\/\">Big Tuna<\/a>\u3092<a href=\"https:\/\/unchainedlabs.com\/jp\/uncle\/\">Uncle<\/a>\u3068\u9023\u643a\u3055\u305b\u308c\u3070\u3001\u5927\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u3053\u308c\u3089\u306e\u6761\u4ef6\u3092\u5e45\u5e83\u304f\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3069\u3061\u3089\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u88c5\u7f6e\u3082\u3001\u30ad\u30e3\u30d7\u30b7\u30c9\u3092\u7834\u58ca\u3059\u308b\u3053\u3068\u306a\u304f\u3001AAV\u3092\u69d8\u3005\u306a\u6761\u4ef6\u4e0b\u3067\u7a4f\u3084\u304b\u306b\u4ea4\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001Uncle\u3092\u4f7f\u7528\u3057\u3066\u3001\u30b2\u30ce\u30e0\u306e\u653e\u51fa\u958b\u59cb\u6e29\u5ea6\u306b\u57fa\u3065\u3044\u3066\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/capsid-stability\/\">AAV\u30ad\u30e3\u30d7\u30b7\u30c9\u306e\u5b89\u5b9a\u6027<\/a>\u3092\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/Get-your-AAV-to-its-happy-place_R2.png\" alt=\"Get your AAV to its happy place_R2\" itemprop=\"image\" height=\"1500\" width=\"2560\" title=\"Get your AAV to its happy place_R2\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t<h3>Unagi<\/h3>\t\t\t\n\t\t\t\t<p>\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u4ea4\u63db\u3068\u6fc3\u7e2e\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u6271\u3046\u524d\u306b\u3084\u3089\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u6700\u3082\u5384\u4ecb\u306a\u3053\u3068\u3067\u3059\u3002\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u7269\u7406\u7684\u306b\u305d\u306e\u5834\u306b\u62d8\u675f\u3055\u308c\u3001\u3084\u3063\u3068\u7d42\u308f\u3063\u305f\u3068\u601d\u3063\u305f\u3089\u307e\u305f\u5fae\u8abf\u6574\u3092\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002Unagi\u306f\u3001\u3053\u306e\u7169\u96d1\u306a\u4f5c\u696d\u3092\u8efd\u6e1b\u3059\u308b\u305f\u3081\u306b\u4f5c\u3089\u308c\u305f\u3001\u30cf\u30f3\u30ba\u30d5\u30ea\u30fc\u306a\u30d9\u30f3\u30c1\u30c8\u30c3 \u30d7\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3067\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\" target=\"_self\" role=\"button\" aria-label=\"UNAGI\u3092\u77e5\u308b\" rel=\"noopener\">\n\t\t\t\t\t\t\tUNAGI\u3092\u77e5\u308b\n\t\t<\/a>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/04\/Unagi-Front_Unagi-section_smaller.png\" alt=\"Unagi-Front_Unagi-section_smaller\" title=\"Unagi-Front_Unagi-section_smaller\" itemprop=\"image\"\/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Big-Tuna-front_crop.png\" alt=\"Big Tuna front_crop\" title=\"Big Tuna front_crop\" itemprop=\"image\"\/>\n\t\t\t<h3>Big Tuna<\/h3>\t\t\t\n\t\t\t\t<p>\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u3068\u30b5\u30f3\u30d7\u30eb\u6fc3\u7e2e\u306f\u3001\u672c\u5f53\u306b\u3084\u308a\u305f\u3044\u3053\u3068\u3092\u3059\u308b\u524d\u306b\u3001\u6642\u9593\u3068\u624b\u9593\u306e\u304b\u304b\u308b\u7169\u96d1\u306a\u4f5c\u696d\u3067\u3059\u3002Big Tuna\u306f\u81ea\u52d5\u5316\u3055\u308c\u305f\u30d7\u30ec\u30fc\u30c8\u30d9\u30fc\u30b9\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3001\u6539\u826f\u3055\u308c\u305fUF\/DF\u6cd5\u3068\u7a4f\u3084\u304b\u306a\u6df7\u5408\u306b\u3088\u308a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u6838\u9178\u3001\u307e\u305f\u306fAAV\u3084LNP\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u6cbb\u7642\u30d9\u30af\u30bf\u30fc\u306a\u3069\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u3001\u6fc3\u7e2e\u3001\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3057\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u524d\u51e6\u7406\u306b\u304b\u304b\u308b\u6642\u9593\u3068\u624b\u9593\u3092\u7701\u304d\u3001\u4ed6\u306e\u91cd\u8981\u306a\u4f5c\u696d\u306b\u5c02\u5ff5\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/big-tuna\/\" target=\"_self\" role=\"button\" aria-label=\"BIG TUNA\u306e\u8a73\u7d30\" rel=\"noopener\">\n\t\t\t\t\t\t\tBIG TUNA\u306e\u8a73\u7d30\n\t\t<\/a>\n<h2>\n\t\t\u3082\u3063\u3068\u8a73\u3057\u304f\u77e5\u308a\u305f\u3044\u3067\u3059\u304b\uff1f\n\t<\/h2>\n\t<p>Unagi \u3068 Big Tuna\u304c\u3069\u306e\u3088\u3046\u306bAAV\u306e\u6fc3\u7e2e\u3068\u30ea\u30d5\u30a9\u30fc\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u7c21\u5358\u306b\u3059\u308b\u304b\u3001\u3082\u3063\u3068\u77e5\u308a\u305f\u3044\u3067\u3059\u304b\uff1f<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u304a\u554f\u3044\u5408\u308f\u305b\n\t\t\t\t\t<\/a>\n<h2>\n\t\t\u95a2\u9023\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\n\t<\/h2>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/capsid-stability\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u30ad\u30e3\u30d7\u30b7\u30c9\u306e\u5b89\u5b9a\u6027\n\t\t\t\t\t<\/a>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/titer-empty-full-ratio\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u529b\u4fa1\uff06Empty\/Full\u6bd4\u7387\n\t\t\t\t\t<\/a>\n\n","protected":false},"excerpt":{"rendered":"<p>AAV \u306e\u6fc3\u7e2e\u3068\u30ea\u30d5\u30a9\u30fc\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3 \u8ab2\u984c \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 \u5b9f\u8a3c\u30c7\u30fc\u30bf \u95a2\u9023\u88fd\u54c1 &#8211; Unagi \u95a2\u9023\u88fd\u54c1 &#8211; Big Tuna \u8ab2\u984c AAV\u306f\u5927\u62b5\u306e\u5834\u5408\u4f4e\u6fc3\u5ea6\u3067\u751f\u7523\u3055\u308c\u308b\u305f\u3081\u3001\u305d\u306e\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3068\u3068\u6fc3\u7e2e\u304c\u5fc5\u8981\u3067\u3059\u3002\u9060\u5fc3\u6ffe\u904e\u88c5\u7f6e\u30841\u56de1\u56de\u306e\u30d5\u30ed\u30fc\u6ffe\u904e\u88c5\u7f6e\u3067\u3053\u306e\u30d7\u30ed\u30bb\u30b9\u306b\u53d6\u308a\u7d44\u3080\u306e\u306f\u3001\u7279\u306b\u30b5\u30f3\u30d7\u30eb\u6570\u3084\u91cf\u304c\u591a\u3044\u5834\u5408\u3001\u624b\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u3002AAV\u306e\u3088\u3046\u306a\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5b8c\u5168\u6027\u3092\u640d\u306a\u308f\u305a\u3001\u304b\u3064\u9ad8\u3044\u56de\u53ce\u7387\u3092\u6301\u3064\u8fc5\u901f\u306a\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3068\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u304c\u5fc5\u8981\u3067\u3059\u3002 \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 Unagi\u306f\u3001AAV\u30b5\u30f3\u30d7\u30eb\u306e\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3068\u6fc3\u7e2e\u306b\u5fc5\u8981\u306a\u3001\u624b\u9593\u306e\u304b\u304b\u308b\u3059\u3079\u3066\u306e\u30b9\u30c6\u30c3\u30d7\u3092\uff11\u3064\u306b\u307e\u3068\u3081\u305f\u521d\u306e\u30d9\u30f3\u30c1\u30c8\u30c3\u30d7\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002Unagi\u306f\u9650\u5916\u308d\u904e\/\u30c0\u30a4\u30a2\u30d5\u30a3\u30eb\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\uff08UF\/DF\uff09\u3092\u4f7f\u7528\u3057\u3066\u30010.5\uff5e8mL\u306e\u958b\u59cb\u5bb9\u91cf\u304b\u30891\uff5e8\u30b5\u30f3\u30d7\u30eb\u307e\u3067\u4ea4\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Unagi\u306f\u5b9f\u884c\u4e2d\u306b\u30b5\u30f3\u30d7\u30eb\u306e\u5bb9\u91cf\u3092\u6ce8\u610f\u6df1\u304f\u30e2\u30cb\u30bf\u30fc\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u308d\u904e\u3057\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u3082\u3069\u3059\u3079\u304d\u5bb9\u91cf\u3092\u628a\u63e1\u3057\u3066\u3044\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u304c\u76ee\u6a19\u306e\u4ea4\u63db\u7387\u307e\u305f\u306fAAV\u6fc3\u5ea6\u306b\u9054\u3059\u308b\u307e\u3067\u3001\u3053\u308c\u3092\u7e70\u308a\u8fd4\u3057\u307e\u3059\u3002 Unagi \u306fUna\u3068\u547c\u3070\u308c\u308b\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u5404Una\u306f0.5\uff5e8mL\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u4fdd\u6301\u3057\u3001Una\u306f1\u30e9\u30f3\u3042\u305f\u308a1\uff5e8\u30b5\u30f3\u30d7\u30eb\u3092\u53d6\u308a\u6271\u3044\u307e\u3059\u3002\u5206\u5b50\u91cf10\u300130\u3001100kDa\u306e\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u306f\u5438\u53ce\u3055\u308c\u308b\u3053\u3068\u306a\u304f\u300196\uff05\u4ee5\u4e0a\u306e\u56de\u53ce\u7387\u3067\u6db2\u4f53\u3092\u901a\u904e\u3057\u307e\u3059\u3002 Big Tuna\u306f\u3001\u3088\u308a\u5747\u4e00\u306a\u30b5\u30f3\u30d7\u30eb\u30cf\u30f3\u30c9\u30ea\u30f3\u30b0\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u3068\u3001\u30de\u30cb\u30e5\u30a2\u30eb\u6cd5\u3067\u306f\u5f97\u3089\u308c\u306a\u3044\u9ad8\u5ea6\u306a\u30d7\u30ed\u30bb\u30b9\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u63d0\u4f9b\u3059\u308b\u5b8c\u5168\u81ea\u52d5\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002Big Tuna\u306f\u3001\u6539\u826f\u578b\u9650\u5916\u308d\u904e\/\u900f\u6790\u308d\u904e\uff08UF\/DF\uff09\u6cd5\u3092\u7528\u3044\u3066\u3001AAV\u3084\u305d\u306e\u4ed6\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3092\u4ea4\u63db\u30fb\u6fc3\u7e2e\u3057\u307e\u3059\u3002Big Tuna\u306f\u3001\u904b\u8ee2\u4e2d\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u30e2\u30cb\u30bf\u30fc\u3057\u3001\u308d\u904e\u3055\u308c\u305f\u91cf\u3092\u623b\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u6bce\u56de\u9069\u5207\u306a\u4ea4\u63db\u7387\u3068\u6fc3\u5ea6\u306b\u3057\u307e\u3059\u3002 Big Tuna\u3067\u306f\u3001Unfilter\u3068\u547c\u3070\u308c\u308b\u30d7\u30ec\u30fc\u30c8\u30d9\u30fc\u30b9\u306e\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u3059\u3079\u3066\u306eUnfilter\u306f\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3092\u4f7f\u7528\u3057\u3066\u304a\u308a\u3001\u975e\u7279\u7570\u7684\u7d50\u5408\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u6700\u9069\u3067\u3059\u300224\u30a6\u30a7\u30eb\uff080.45 &#8211; 8 mL\uff09\u306896\u30a6\u30a7\u30eb\uff08100 &#8211; 450 \u00b5L\uff09\u306e2\u3064\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\u3002Unfilter 96\u306f\u3001\u5206\u5b50\u91cf\u30ab\u30c3\u30c8\u30aa\u30d5\u304c3\u300110\u300130\u3001100 kDa\u3002Unfilter 24\u306e\u5206\u5b50\u91cf\u306f10\u300130\u3001100 kDa\u3067\u3059\u3002\u5206\u5b50\u91cf\u306e\u9ad8\u3044\u30ab\u30c3\u30c8\u30aa\u30d5\u5024\u306f\u3001\u5927\u304d\u306a\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u6700\u9069\u3067\u3059\u3002Unfilter 24 \u306f\u6700\u5927 48 mL \u3092 8 mL \u307e\u3067\u6fc3\u7e2e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u5b9f\u8a3c\u30c7\u30fc\u30bf AAV\u30b5\u30f3\u30d7\u30eb\u306e\u6700\u9069\u5316\u3068\u5b89\u5b9a\u6027\u8a55\u4fa1 AAV\u306e\u5b89\u5b9a\u6027\u306f\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u306epH\u3001\u5869\u3001\u30a4\u30aa\u30f3\u5f37\u5ea6\u306e\u5909\u5316\u306b\u3088\u3063\u3066\u5f71\u97ff\u3092\u53d7\u3051\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002Unagi\u307e\u305f\u306fBig Tuna\u3092Uncle\u3068\u9023\u643a\u3055\u305b\u308c\u3070\u3001\u5927\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u3053\u308c\u3089\u306e\u6761\u4ef6\u3092\u5e45\u5e83\u304f\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3069\u3061\u3089\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u88c5\u7f6e\u3082\u3001\u30ad\u30e3\u30d7\u30b7\u30c9\u3092\u7834\u58ca\u3059\u308b\u3053\u3068\u306a\u304f\u3001AAV\u3092\u69d8\u3005\u306a\u6761\u4ef6\u4e0b\u3067\u7a4f\u3084\u304b\u306b\u4ea4\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001Uncle\u3092\u4f7f\u7528\u3057\u3066\u3001\u30b2\u30ce\u30e0\u306e\u653e\u51fa\u958b\u59cb\u6e29\u5ea6\u306b\u57fa\u3065\u3044\u3066\u3001AAV\u30ad\u30e3\u30d7\u30b7\u30c9\u306e\u5b89\u5b9a\u6027\u3092\u8a55\u4fa1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 Unagi \u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u4ea4\u63db\u3068\u6fc3\u7e2e\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u6271\u3046\u524d\u306b\u3084\u3089\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u6700\u3082\u5384\u4ecb\u306a\u3053\u3068\u3067\u3059\u3002\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u7269\u7406\u7684\u306b\u305d\u306e\u5834\u306b\u62d8\u675f\u3055\u308c\u3001\u3084\u3063\u3068\u7d42\u308f\u3063\u305f\u3068\u601d\u3063\u305f\u3089\u307e\u305f\u5fae\u8abf\u6574\u3092\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002Unagi\u306f\u3001\u3053\u306e\u7169\u96d1\u306a\u4f5c\u696d\u3092\u8efd\u6e1b\u3059\u308b\u305f\u3081\u306b\u4f5c\u3089\u308c\u305f\u3001\u30cf\u30f3\u30ba\u30d5\u30ea\u30fc\u306a\u30d9\u30f3\u30c1\u30c8\u30c3 \u30d7\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3067\u3059\u3002 UNAGI\u3092\u77e5\u308b Big Tuna \u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u3068\u30b5\u30f3\u30d7\u30eb\u6fc3\u7e2e\u306f\u3001\u672c\u5f53\u306b\u3084\u308a\u305f\u3044\u3053\u3068\u3092\u3059\u308b\u524d\u306b\u3001\u6642\u9593\u3068\u624b\u9593\u306e\u304b\u304b\u308b\u7169\u96d1\u306a\u4f5c\u696d\u3067\u3059\u3002Big 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