{"id":5079,"date":"2021-12-08T07:04:50","date_gmt":"2021-12-08T07:04:50","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=5079"},"modified":"2024-05-17T04:55:27","modified_gmt":"2024-05-17T04:55:27","slug":"quantification-sizing","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/quantification-sizing\/","title":{"rendered":"LNP quantification &#038; sizing"},"content":{"rendered":"\n<h1>\n\t\tLNP\u5b9a\u91cf\u3068\u30b5\u30a4\u30b8\u30f3\u30b0\n\t<\/h1>\n\t<ul>\n<li><a href=\"#problem\">\u8ab2\u984c<\/a><\/li>\n<li><a href=\"#solution\">\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/a><\/li>\n<li><a href=\"#proof\">\u5b9f\u8a3c\u30c7\u30fc\u30bf<\/a><\/li>\n<li><a href=\"#stunner\">\u95a2\u9023\u88fd\u54c1 <strong>&#8211; Stunner<\/strong><\/a><\/li>\n<\/ul>\n<h4>\n\t\t\u8ab2\u984c\n\t<\/h4>\n\t<p>\u30ca\u30ce\u7c92\u5b50\u7528\u9014\u3067\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u30da\u30a4\u30ed\u30fc\u30c9\u3068\u3057\u3066\u3001\u4f8b\u3048\u3070\u3001RNA\u3001DNA\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002\u30da\u30a4\u30ed\u30fc\u30c9\u306e\u5b9a\u91cf\u306b\u95a2\u3057\u3066\u306f\u3001\u307b\u3068\u3093\u3069\u306e\u6eb6\u6db2\u3067\u306f\u3001LNP\u3068\u30ca\u30ce\u7c92\u5b50\u306e\u6fc1\u308a\u3092\u56de\u907f\u3059\u308b\u305f\u3081\u306b\u8907\u96d1\u306a\u30a2\u30c3\u30bb\u30a4\u304c\u5fc5\u8981\u3067\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306f\u6fc1\u3063\u3066\u3044\u308b\u305f\u3081\u3001\u5b9a\u91cf\u306b\u306f\u86cd\u5149\u8272\u7d20\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u304c\u3001\u30ca\u30ce\u7c92\u5b50\u5206\u6790\u306b\u306f\u6271\u3044\u306b\u304f\u3044\u8272\u7d20\u30d9\u30fc\u30b9\u306e\u5206\u6790\u6cd5\u306f\u3042\u307e\u308a\u597d\u307e\u308c\u307e\u305b\u3093\u3002<\/p>\n<p>UV\/Vis\u5438\u5149\u5ea6\u6cd5\u306f\u3001\u7cbe\u88fd\u30b5\u30f3\u30d7\u30eb\u4e2d\u306eRNA\u5b9a\u91cf\u306e\u305f\u3081\u306e\u8fc5\u901f\u3001\u7c21\u5358\u3001\u4f4e\u30dc\u30ea\u30e5\u30fc\u30e0\u306e\u65b9\u6cd5\u3068\u3057\u3066\u6614\u304b\u3089\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u30ca\u30ce\u7c92\u5b50\u30b5\u30f3\u30d7\u30eb\u306f\u3001\u305d\u306e\u6fc1\u308a\u306b\u3088\u308a\u307b\u3068\u3093\u3069\u306eUV\/Vis\u5206\u5149\u5149\u5ea6\u8a08\u3067\u6b63\u78ba\u306a\u30ca\u30ce\u7c92\u5b50\u5206\u6790\u306e\u8aad\u307f\u53d6\u308a\u3092\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u5b9a\u91cf\u3060\u3051\u3067\u306a\u304f\u3001\u7c92\u5b50\u3092\u9069\u5207\u306a\u30b5\u30a4\u30ba\u306b\u4fdd\u3064\u3053\u3068\u304c\u6210\u529f\u306e\u9375\u3068\u306a\u308b\u305f\u3081\u3001\u30b5\u30a4\u30b8\u30f3\u30b0\u30c7\u30fc\u30bf\u306e\u7372\u5f97\u304c\u3059\u3079\u3066\u306e\u4eba\u306e\u76ee\u6a19\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u3059\u3079\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u30b5\u30a4\u30ba\u30c7\u30fc\u30bf\u304c\u5fc5\u8981\u306a\u5834\u5408\u3001\u591a\u304f\u306e\u52d5\u7684\u5149\u6563\u4e71(DLS)\u88c5\u7f6e\u306f\u30b5\u30f3\u30d7\u30eb\u3092\u4e00\u5ea6\u306b1\u3064\u305a\u3064\u3057\u304b\u6e2c\u5b9a\u3067\u304d\u307es\u306d\u3002<\/p>\n<h4>\n\t\t\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\n\t<\/h4>\n\t<p><a href=\"https:\/\/www.unchainedlabs.com\/jp\/stunner\/\">Stunner<\/a>\u306f\u3001\u5149\u8def\u9577\u306e\u77ed\u3044<a href=\"https:\/\/www.unchainedlabs.com\/jp\/uv-vis-spectroscopy\/\">UV\/Vis<\/a>\u3068\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8<a href=\"https:\/\/unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">RADLS<\/a>\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u3001\u6fc1\u3063\u305f\u30ca\u30ce\u7c92\u5b50\u30b5\u30f3\u30d7\u30eb\u3067\u3082\u3001\u30c8\u30fc\u30bf\u30ebRNA\u3068\u30b5\u30a4\u30ba\u3092\u540c\u6642\u306b\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Stunner\u306f\u3001\u5f53\u793e\u306e\u30ca\u30ce\u7c92\u5b50\u5206\u6790\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3092\u4f7f\u7528\u3057\u3066\u3001\u8a66\u85ac\u3001\u6a19\u6e96\u7269\u8cea\u3001\u86cd\u5149\u8272\u7d20\u3092\u5fc5\u8981\u3068\u305b\u305a\u306b\u3001RNA\u3001DNA\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u307e\u305f\u306f\u4f4e\u5206\u5b50\u306e\u7dcf\u30da\u30a4\u30ed\u30fc\u30c9\u6fc3\u5ea6\u3092\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u72ec\u7279\u306a\u30ca\u30ce\u7c92\u5b50\u306e\u5834\u5408\u3001Stunner\u306f\u3001\u30ad\u30e3\u30ea\u30a2\u304b\u3089\u30da\u30a4\u30ed\u30fc\u30c9\u307e\u3067\u3001\u7c92\u5b50\u306eUV\/Vis\u5438\u5149\u5ea6\u306b\u95a2\u3059\u308b\u3059\u3079\u3066\u3092\u628a\u63e1\u3057\u3001\u8a18\u61b6\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u30022\u03bcL\u306e\u30b5\u30f3\u30d7\u30eb\u3055\u3048\u3042\u308c\u3070\u3001Stunner\u306fRADLS\u30c7\u30fc\u30bf\u3092\u63d0\u4f9b\u3057\u3001\u5fc5\u8981\u306a\u30b5\u30a4\u30ba\u3068PDI\u30c7\u30fc\u30bf\u3092\u53d6\u5f97\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u3089\u306f\u3059\u3079\u3066\u300196\u30a6\u30a7\u30eb\u30de\u30a4\u30af\u30ed\u6d41\u4f53\u30d7\u30ec\u30fc\u30c8\u3067\u30b5\u30f3\u30d7\u30eb\u3042\u305f\u308a1\u5206\u4ee5\u5185\u306b\u5b8c\u6210\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2024\/05\/stunner_workflow_LNP.png\" alt=\"stunner_workflow_LNP\" itemprop=\"image\" height=\"565\" width=\"865\" title=\"stunner_workflow_LNP\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\t\u5b9f\u8a3c\u30c7\u30fc\u30bf\n\t<\/h4>\n<h5>\n\t\t\u8a66\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\n\t<\/h5>\n\t<p>LNP \u306f\u5de8\u5927\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u6563\u4e71\u52b9\u679c\u304c\u3042\u308a\u307e\u3059\u304c\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/stunner\/\">Stunner<\/a> \u306e\u72ec\u81ea\u306e\u56de\u8ee2\u89d2\u5ea6 DLS \u306b\u306f\u6575\u3044\u307e\u305b\u3093\u3002 <a href=\"https:\/\/unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">RADLS<\/a> \u306f\u3001\u30ca\u30ce\u7c92\u5b50\u30b5\u30f3\u30d7\u30eb\u306e\u89d2\u5ea6\u306b\u4f9d\u5b58\u3057\u306a\u3044\u30b5\u30a4\u30b8\u30f3\u30b0\u3068\u5149\u6563\u4e71\u5f37\u5ea6\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u7c92\u5b50\u6fc3\u5ea6\u306e\u53d6\u5f97\u304c\u304b\u3064\u3066\u306a\u3044\u307b\u3069\u7c21\u5358\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2024\/05\/Take-it-for-a-spin_web.png\" alt=\"Take-it-for-a-spin_web\" itemprop=\"image\" height=\"474\" width=\"800\" title=\"Take-it-for-a-spin_web\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\t\u4e00\u7dd2\u306b\u96c6\u3081\u307e\u3057\u3087\u3046\n\t<\/h4>\n\t<p>Stunner \u306e <a href=\"https:\/\/unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">RADLS<\/a> \u3068 <a href=\"https:\/\/unchainedlabs.com\/jp\/uv-vis-spectroscopy\/\">UV\/Vis<\/a> \u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u30b5\u30a4\u30ba\u3001RNA \u6fc3\u5ea6\u3001\u30ca\u30ce\u7c92\u5b50\u6fc3\u5ea6\u306e\u30c7\u30fc\u30bf\u3092\u307e\u3068\u3081\u3066\u3001\u7c92\u5b50\u3042\u305f\u308a\u306e RNA \u5206\u5b50\u306e\u5e73\u5747\u91cf\u3092\u5831\u544a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3055\u307e\u3056\u307e\u306a RNA \u69cb\u7bc9\u7269\u3001\u8102\u8cea\u914d\u5408\u7269\u3001\u307e\u305f\u306f\u30d7\u30ed\u30bb\u30b9\u6761\u4ef6\u3092\u5149\u901f\u3067\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2024\/05\/Get-it-together_web-1.png\" alt=\"Get-it-together_web-1\" itemprop=\"image\" height=\"621\" width=\"800\" title=\"Get-it-together_web-1\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Stunner-Front-cc_crop_downsize.png\" alt=\"Stunner Front cc_crop_downsize\" title=\"Stunner Front cc_crop_downsize\" itemprop=\"image\"\/>\n\t\t\t<h3>Stunner<\/h3>\t\t\t\n\t\t\t\t<p>Stunner\u306f\u30012\u03bcL\u306e\u30b5\u30f3\u30d7\u30eb\u304b\u3089<a href=\"https:\/\/unchainedlabs.com\/jp\/uv-vis-spectroscopy\/\">UV\/Vis<\/a>\u6fc3\u5ea6\u3001<a href=\"https:\/\/unchainedlabs.com\/jp\/rotating-angle-dynamic-light-scattering\/\">\u56de\u8ee2\u89d2\u52d5\u7684\u5149\u6563\u4e71<\/a>\uff08RADLS\uff09\u3001\u591a\u89d2\u5ea6\u5149\u6563\u4e71\uff08MALS\uff09\u30c7\u30fc\u30bf\u3092\u53d6\u5f97\u3067\u304d\u308b\u552f\u4e00\u306e\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u304cLNP\u3067\u3042\u308c\u3070\u3001\u305d\u306e\u7c92\u5b50\u6fc3\u5ea6\u3001\u30b5\u30a4\u30ba\u3001\u8cea\u91cf\u3001\u51dd\u96c6\u4f53\u306e\u691c\u51fa\u3001\u304a\u3088\u3073\u7dcfRNA\u3092\u30c1\u30a7\u30c3\u30af\u3057\u3001\u30ca\u30ce\u7c92\u5b50\u306e\u54c1\u8cea\u3092\u78ba\u5b9a\u3055\u305b\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308c\u3070\u3001\u6fc3\u5ea6\u3001\u30b5\u30a4\u30b8\u30f3\u30b0\u3001\u5206\u5b50\u91cf\u3001\u304a\u3088\u3073\u51dd\u96c6\u4f53\u306e\u691c\u51fa\u3092\u4e00\u5ea6\u306b\u884c\u3044\u307e\u3059\u3002AAV\u307e\u305f\u306f\u30a2\u30c7\u30ce\u30a6\u30a4\u30eb\u30b9\u3067\u3042\u308c\u3070\u3001\u7dcf\u30ad\u30e3\u30d7\u30b7\u30c9\u529b\u4fa1\u3068Empty\/Full\u6bd4\u3092\u628a\u63e1\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u5373\u5ea7\u306b\u304a\u6301\u3061\u306eLNP\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u307e\u305f\u306f\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306e\u54c1\u8cea\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/stunner\/\" target=\"_self\" role=\"button\" aria-label=\"STUNNER\u95a2\u9023\u60c5\u5831\" rel=\"noopener\">\n\t\t\t\t\t\t\tSTUNNER\u95a2\u9023\u60c5\u5831\n\t\t<\/a>\n<h2>\n\t\t\u3055\u3089\u306b\u8a73\u3057\u3044\u60c5\u5831\u306f\u3053\u3061\u3089\n\t<\/h2>\n\t<p>\u30ca\u30ce\u7c92\u5b50\u306e\u5b9a\u91cf\u3068\u30b5\u30a4\u30b8\u30f3\u30b0\u304c\u3067\u304d\u308bStunner\u306b\u3064\u3044\u3066\u3001\u3082\u3063\u3068\u77e5\u308a\u305f\u3044\u3067\u3059\u304b\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u304a\u554f\u3044\u5408\u308f\u305b\n\t\t\t\t\t<\/a>\n<h2>\n\t\t\u95a2\u9023\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\n\t<\/h2>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/nucleic-acid-quantification\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u6838\u9178\u5b9a\u91cf\n\t\t\t\t\t<\/a>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/clean-up-concentration\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\uff06\u6fc3\u7e2e\n\t\t\t\t\t<\/a>\n\n","protected":false},"excerpt":{"rendered":"<p>LNP\u5b9a\u91cf\u3068\u30b5\u30a4\u30b8\u30f3\u30b0 \u8ab2\u984c \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 \u5b9f\u8a3c\u30c7\u30fc\u30bf \u95a2\u9023\u88fd\u54c1 &#8211; Stunner \u8ab2\u984c \u30ca\u30ce\u7c92\u5b50\u7528\u9014\u3067\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u30da\u30a4\u30ed\u30fc\u30c9\u3068\u3057\u3066\u3001\u4f8b\u3048\u3070\u3001RNA\u3001DNA\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\u304c\u3042\u308a\u307e\u3059\u3002\u30da\u30a4\u30ed\u30fc\u30c9\u306e\u5b9a\u91cf\u306b\u95a2\u3057\u3066\u306f\u3001\u307b\u3068\u3093\u3069\u306e\u6eb6\u6db2\u3067\u306f\u3001LNP\u3068\u30ca\u30ce\u7c92\u5b50\u306e\u6fc1\u308a\u3092\u56de\u907f\u3059\u308b\u305f\u3081\u306b\u8907\u96d1\u306a\u30a2\u30c3\u30bb\u30a4\u304c\u5fc5\u8981\u3067\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306f\u6fc1\u3063\u3066\u3044\u308b\u305f\u3081\u3001\u5b9a\u91cf\u306b\u306f\u86cd\u5149\u8272\u7d20\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u304c\u3001\u30ca\u30ce\u7c92\u5b50\u5206\u6790\u306b\u306f\u6271\u3044\u306b\u304f\u3044\u8272\u7d20\u30d9\u30fc\u30b9\u306e\u5206\u6790\u6cd5\u306f\u3042\u307e\u308a\u597d\u307e\u308c\u307e\u305b\u3093\u3002 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