{"id":5110,"date":"2021-12-08T07:45:35","date_gmt":"2021-12-08T07:45:35","guid":{"rendered":"http:\/\/unchainedlabs.sheldon.com\/?page_id=5110"},"modified":"2024-05-17T04:56:31","modified_gmt":"2024-05-17T04:56:31","slug":"clean-up-concentration","status":"publish","type":"page","link":"https:\/\/unchainedlabs.com\/jp\/clean-up-concentration\/","title":{"rendered":"LNP clean-up &#038; concentration"},"content":{"rendered":"\n<h1>\n\t\t\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\uff06\u6fc3\u7e2e\n\t<\/h1>\n\t<ul>\n<li><a href=\"#problem\">\u8ab2\u984c<\/a><\/li>\n<li><a href=\"#solution\">\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/a><\/li>\n<li><a href=\"#proof\">\u5b9f\u8a3c\u30c7\u30fc\u30bf<\/a><\/li>\n<li><a href=\"#unagi\">\u95a2\u9023\u88fd\u54c1 <strong>&#8211; Unagi<\/strong><\/a><\/li>\n<li><a href=\"#big_tuna\">\u95a2\u9023\u88fd\u54c1 <strong>&#8211; Big Tuna<\/strong><\/a><\/li>\n<\/ul>\n<h4>\n\t\t\u8ab2\u984c\n\t<\/h4>\n\t<p>\u8102\u8cea\u30ca\u30ce\u7c92\u5b50\uff08LNP\uff09\u304c\u5f62\u6210\u3055\u308c\u305f\u5f8c\u3001\u85ac\u7269\u306e\u5c01\u5165\u3068LNP\u306e\u5b8c\u5168\u6027\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u306a\u3069\u306e\u6709\u6a5f\u6eb6\u5a92\u3092\u8fc5\u901f\u306b\u9664\u53bb\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u900f\u6790\u304c\u3088\u304f\u7528\u3044\u3089\u308c\u307e\u3059\u304c\u3001\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u6e1b\u3089\u3059\u305f\u3081\u306b\u4e8c\u6b21\u6fc3\u7e2e\u306e\u30b9\u30c6\u30c3\u30d7\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n<h4>\n\t\t\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\n\t<\/h4>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\">Unagi<\/a>\u306f\u3001\u5b8c\u5168\u6027\u3092\u640d\u306a\u3046\u3053\u3068\u306a\u304f\u3001LNP\u306e\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3001\u6fc3\u7e2e\u3001\u4ea4\u63db\u306b\u5fc5\u8981\u306a\u3059\u3079\u3066\u306e\u624b\u9593\u306e\u304b\u304b\u308b\u5b9f\u8df5\u7684\u306a\u30b9\u30c6\u30c3\u30d7\u3092\u307e\u3068\u3081\u305f\u521d\u306e\u30d9\u30f3\u30c1\u30c8\u30c3\u30d7\u578b\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002<a href=\"https:\/\/unchainedlabs.com\/jp\/ultrafiltration-diafiltration-uf-df\/\">\u9650\u5916\u6ffe\u904e\u6cd5\/\u30c0\u30a4\u30a2\u30d5\u30a3\u30eb\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\uff08UF\/DF\uff09<\/a>\u3092\u7528\u3044\u3066\u30010.5\uff5e8mL\u306e\u958b\u59cb\u5bb9\u91cf\u304b\u30891\uff5e8\u30b5\u30f3\u30d7\u30eb\u3092\u4ea4\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Unagi\u306f\u3001\u5b9f\u884c\u4e2d\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u6ce8\u610f\u6df1\u304f\u8ffd\u8de1\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u308d\u904e\u3057\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u8ffd\u52a0\u3059\u308b\u91cf\u3092\u6b63\u78ba\u306b\u628a\u63e1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u4ea4\u63db\u7387\u307e\u305f\u306f\u6fc3\u5ea6\u306b\u9054\u3059\u308b\u307e\u3067\u3001\u3053\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u7e70\u308a\u8fd4\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/03\/The-secret-sauce.png\" alt=\"The secret sauce\" itemprop=\"image\" height=\"281\" width=\"828\" title=\"The secret sauce\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\">Unagi<\/a>\u306fUna\u3068\u3044\u3046\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u5404Una\u306b\u306f0.5\uff5e8mL\u306e\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u308a\u30011\u56de\u306e\u5206\u6790\u30671\uff5e8\u500b\u306eUna\u304c\u51e6\u7406\u3055\u308c\u307e\u3059\u3002\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\uff08\u5206\u5b50\u91cf10\u300130\u3001100 kDa MWCO\uff09\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u306f\u3088\u304f\u4fdd\u6301\u3055\u308c\u3001\u6db2\u4f53\u306f\u5438\u53ce\u3055\u308c\u305a\u306b\u901a\u904e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u6db2\u4f53\u306e\u56de\u53ce\u7387\u306f\u226596%\u3092\u8a87\u308a\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/04\/Served-up-ala-carte-2.png\" alt=\"Served-up-ala-carte-2\" itemprop=\"image\" height=\"529\" width=\"900\" title=\"Served-up-ala-carte-2\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/www.unchainedlabs.com\/jp\/big-tuna\">Big Tuna<\/a>\u306fLNP\u306e\u8fc5\u901f\u306a\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3001\u6fc3\u7e2e\u3001\u4ea4\u63db\u306e\u305f\u3081\u306b\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/ultrafiltration-diafiltration-uf-df\">\u9650\u5916\u6ffe\u904e\u6cd5\/\u30c0\u30a4\u30a2\u30d5\u30a3\u30eb\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\uff08UF\/DF\uff09<\/a>\u3092\u4f7f\u7528\u3057\u3066\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3092\u5411\u4e0a\u3055\u305b\u308b\u5b8c\u5168\u81ea\u52d5\u5316\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u7a3c\u50cd\u4e2d\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u30e2\u30cb\u30bf\u30fc\u3057\u3001\u308d\u904e\u3055\u308c\u305f\u91cf\u3092\u623b\u3057\u3001\u6bce\u56de\u9069\u5207\u306a\u6bd4\u7387\u306e\u4ea4\u63db\u3068\u6fc3\u7e2e\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002LNP\u306e\u6709\u6a5f\u6eb6\u5a92\u306f\u901a\u5e38\u6025\u901f\u306b\u6d41\u308c\u307e\u3059\u304c\u3001Big Tuna\u306f\u9069\u5207\u306a\u8abf\u6574\u3092\u884c\u3046\u80fd\u529b\u3092\u5099\u3048\u3066\u3044\u308b\u306e\u3067\u3001\u30a6\u30a7\u30eb\u304c\u67af\u6e07\u3059\u308b\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/automated_buffer_exchange_unfilter_only.png\" alt=\"automated_buffer_exchange_unfilter_only\" itemprop=\"image\" height=\"246\" width=\"828\" title=\"automated_buffer_exchange_unfilter_only\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/www.unchainedlabs.com\/jp\/big-tuna\">Big Tuna<\/a>\u3067\u306f\u3001<a href=\"https:\/\/www.unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/45_Unfilter_spec_sheet.pdf\" target=\"_blank\" rel=\"noopener\">Unfilter<\/a>\u3068\u3044\u3046\u30d7\u30ec\u30fc\u30c8\u30d9\u30fc\u30b9\u306e\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u3059\u3079\u3066\u306eUnfilter\u306f\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3092\u4f7f\u7528\u3057\u3066\u304a\u308a\u3001\u975e\u7279\u7570\u7684\u7d50\u5408\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u6700\u9069\u3067\u3059\u300224\u30a6\u30a7\u30eb\uff080.45\uff5e8mL\uff09\u306896\u30a6\u30a7\u30eb\uff08100\uff5e450\u03bcL\uff09\u306e2\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002Unfilter96\u306f\u30013\u300110\u300130\u3001100kDa\u306e\u5206\u5b50\u91cf\u30ab\u30c3\u30c8\u30aa\u30d5\uff08MWCO\uff09\u306b\u5bfe\u5fdc\u3057\u3001Unfilter24\u306f10\u300130\u3001100kDa\u306eMWCO\u306b\u5bfe\u5fdc\u3067\u304d\u307e\u3059\u300224\u30a6\u30a7\u30eb\u3001100kDa\u306eUnfilter\u306f\u3001\u5927\u91cf\u306eLNP\u30b5\u30f3\u30d7\u30eb\u3092\u9ad8\u901f\u3067\u51e6\u7406\u3059\u308b\u306e\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/2plates.png\" alt=\"2plates\" itemprop=\"image\" height=\"259\" width=\"900\" title=\"2plates\" onerror=\"this.style.display='none'\"  \/>\n<h4>\n\t\t\u5b9f\u8a3c\n\t<\/h4>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\">Unagi<\/a>\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u304a\u3088\u3073\u6fc3\u7e2e\u30d1\u30e9\u30e1\u30fc\u30bf\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5b8c\u5168\u6027\u3092\u4fdd\u3061\u306a\u304c\u3089\u3001\u9ad8\u3044\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u7387\u3092\u9054\u6210\u3059\u308b\u305f\u3081\u306b\u6700\u9069\u5316\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002LNP\u30b5\u30f3\u30d7\u30eb\u6e96\u5099\u306b\u304a\u3044\u3066\u3001\u30b5\u30a4\u30ba\u5909\u5316\u3084\u85ac\u7269\u5c01\u5165\u52b9\u7387\u306e\u3088\u3046\u306a\u30b5\u30f3\u30d7\u30eb\u306e\u54c1\u8cea\u3092\u72a0\u7272\u306b\u3059\u308b\u5fc5\u8981\u306a\u304f\u3001\u624b\u9593\u3092\u7bc0\u7d04\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/09\/Unagi-table_2.png\" alt=\"Unagi table_2\" itemprop=\"image\" height=\"309\" width=\"810\" title=\"Unagi table_2\" onerror=\"this.style.display='none'\"  \/>\n\t<p><a href=\"https:\/\/unchainedlabs.com\/jp\/big-tuna\/\">Big Tuna<\/a>\u306f\u3001\u30da\u30a4\u30ed\u30fc\u30c9\u306e\u85ac\u7269\u5c01\u5165\u3092\u7dad\u6301\u3057\u306a\u304c\u3089\u30ca\u30ce\u7c92\u5b50\u306e\u6fc3\u7e2e\u3068\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5178\u578b\u7684\u306a\u30ca\u30ce\u7c92\u5b50\u306e\u6fc3\u7e2e\u3068\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3067\u3057\u305f\u3089\u3001\u6e96\u5099\u6642\u9593\u306f30\u5206\u672a\u6e80\u3067\u3001\u30e9\u30f3\u306f\u6700\u77ed90\u5206\u3067\u7d42\u4e86\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2021\/12\/LNP-clean-up-concentrate-table.jpg\" alt=\"LNP clean up &amp; concentrate table\" itemprop=\"image\" height=\"224\" width=\"787\" title=\"LNP clean up &amp; concentrate table\" onerror=\"this.style.display='none'\"  \/>\n\t\t\t<h3>Unagi<\/h3>\t\t\t\n\t\t\t\t<p>\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u3068\u6fc3\u7e2e\u306f\u3001\u5c11\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u3042\u3063\u3066\u3082\u5fc5\u305a\u884c\u308f\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u7169\u96d1\u306a\u4f5c\u696d\u3067\u3059\u3002\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u76ee\u3092\u96e2\u305b\u305a\u3001\u7d42\u308f\u3063\u305f\u3068\u601d\u3063\u305f\u3089\u307e\u305f\u5fae\u8abf\u6574\u3092\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002Unagi\u3092\u4f7f\u3048\u3070\u3001\u30e9\u30dc\u306b\u5f35\u308a\u4ed8\u304f\u3053\u3068\u306a\u304f\u3001\u30e9\u30f3\u306e\u9593\u306f\u4ed6\u306e\u5834\u6240\u3067\u4f5c\u696d\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/unagi\/\" target=\"_self\" role=\"button\" aria-label=\"UNAGI\u95a2\u9023\u60c5\u5831\" rel=\"noopener\">\n\t\t\t\t\t\t\tUNAGI\u95a2\u9023\u60c5\u5831\n\t\t<\/a>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/04\/Unagi-Front_Unagi-section_smaller-2.png\" alt=\"Unagi-Front_Unagi-section_smaller-2\" title=\"Unagi-Front_Unagi-section_smaller-2\" itemprop=\"image\"\/>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/unchainedlabs.com\/jp\/wp-content\/uploads\/2022\/01\/Big-Tuna-front_crop.png\" alt=\"Big Tuna front_crop\" title=\"Big Tuna front_crop\" itemprop=\"image\"\/>\n\t\t\t<h3>Big Tuna<\/h3>\t\t\t\n\t\t\t\t<p>Big Tuna\u306f\u7169\u308f\u3057\u3044\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30fb\u6fc3\u7e2e\u4f5c\u696d\u3092\u5b8c\u5168\u81ea\u52d5\u5316\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3001AAV\u3001LLP\u3001VLP\u3001VLP\u306a\u3069\u306e\u907a\u4f1d\u5b50\u6cbb\u7642\u30d9\u30af\u30bf\u30fc\u306e\u8abf\u88fd\u3001\u6fc3\u7e2e\u3001\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u306e\u305f\u3081\u306e\u4ea4\u63db\u3092\u308f\u305a\u304b30\u5206\u306e\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u6642\u9593\u3067\u958b\u59cb\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u6e96\u5099\u306b\u304b\u304b\u308b\u6642\u9593\u3068\u624b\u9593\u3092\u7701\u304d\u3001\u4ed6\u306e\u91cd\u8981\u306a\u4f5c\u696d\u306b\u5c02\u5ff5\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/big-tuna\/\" target=\"_self\" role=\"button\" aria-label=\"BIG TUNA \u95a2\u9023\u60c5\u5831\" rel=\"noopener\">\n\t\t\t\t\t\t\tBIG TUNA \u95a2\u9023\u60c5\u5831\n\t\t<\/a>\n<h2>\n\t\t\u3055\u3089\u306b\u8a73\u3057\u3044\u60c5\u5831\u306f\u3053\u3061\u3089\n\t<\/h2>\n\t<p>\u8102\u8cea\u30ca\u30ce\u7c92\u5b50\u306e\u524d\u51e6\u7406\u306b\u5f79\u7acb\u3064Unagi\u3068Big Tuna\u306b\u3064\u3044\u3066\u3082\u3063\u3068\u77e5\u308a\u305f\u3044\u3067\u3059\u304b\u3002<\/p>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/contact-us\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u304a\u554f\u3044\u5408\u308f\u305b\n\t\t\t\t\t<\/a>\n<h2>\n\t\t\u95a2\u9023\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\n\t<\/h2>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/quantification-sizing\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u5b9a\u91cf\u5316\u3068\u30b5\u30a4\u30b8\u30f3\u30b0\n\t\t\t\t\t<\/a>\n\t\t\t<a href=\"https:\/\/unchainedlabs.com\/jp\/nucleic-acid-quantification\/\" target=\"_self\" rel=\"noopener\">\n\t\t\t\t\t\t\t\u6838\u9178\u5b9a\u91cf\n\t\t\t\t\t<\/a>\n\n","protected":false},"excerpt":{"rendered":"<p>\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\uff06\u6fc3\u7e2e \u8ab2\u984c \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 \u5b9f\u8a3c\u30c7\u30fc\u30bf \u95a2\u9023\u88fd\u54c1 &#8211; Unagi \u95a2\u9023\u88fd\u54c1 &#8211; Big Tuna \u8ab2\u984c \u8102\u8cea\u30ca\u30ce\u7c92\u5b50\uff08LNP\uff09\u304c\u5f62\u6210\u3055\u308c\u305f\u5f8c\u3001\u85ac\u7269\u306e\u5c01\u5165\u3068LNP\u306e\u5b8c\u5168\u6027\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u306a\u3069\u306e\u6709\u6a5f\u6eb6\u5a92\u3092\u8fc5\u901f\u306b\u9664\u53bb\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u900f\u6790\u304c\u3088\u304f\u7528\u3044\u3089\u308c\u307e\u3059\u304c\u3001\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u6e1b\u3089\u3059\u305f\u3081\u306b\u4e8c\u6b21\u6fc3\u7e2e\u306e\u30b9\u30c6\u30c3\u30d7\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002 \u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3 Unagi\u306f\u3001\u5b8c\u5168\u6027\u3092\u640d\u306a\u3046\u3053\u3068\u306a\u304f\u3001LNP\u306e\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3001\u6fc3\u7e2e\u3001\u4ea4\u63db\u306b\u5fc5\u8981\u306a\u3059\u3079\u3066\u306e\u624b\u9593\u306e\u304b\u304b\u308b\u5b9f\u8df5\u7684\u306a\u30b9\u30c6\u30c3\u30d7\u3092\u307e\u3068\u3081\u305f\u521d\u306e\u30d9\u30f3\u30c1\u30c8\u30c3\u30d7\u578b\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3067\u3059\u3002\u9650\u5916\u6ffe\u904e\u6cd5\/\u30c0\u30a4\u30a2\u30d5\u30a3\u30eb\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\uff08UF\/DF\uff09\u3092\u7528\u3044\u3066\u30010.5\uff5e8mL\u306e\u958b\u59cb\u5bb9\u91cf\u304b\u30891\uff5e8\u30b5\u30f3\u30d7\u30eb\u3092\u4ea4\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002Unagi\u306f\u3001\u5b9f\u884c\u4e2d\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u6ce8\u610f\u6df1\u304f\u8ffd\u8de1\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u308d\u904e\u3057\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u8ffd\u52a0\u3059\u308b\u91cf\u3092\u6b63\u78ba\u306b\u628a\u63e1\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u4ea4\u63db\u7387\u307e\u305f\u306f\u6fc3\u5ea6\u306b\u9054\u3059\u308b\u307e\u3067\u3001\u3053\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u7e70\u308a\u8fd4\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 Unagi\u306fUna\u3068\u3044\u3046\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u5404Una\u306b\u306f0.5\uff5e8mL\u306e\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u308a\u30011\u56de\u306e\u5206\u6790\u30671\uff5e8\u500b\u306eUna\u304c\u51e6\u7406\u3055\u308c\u307e\u3059\u3002\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\uff08\u5206\u5b50\u91cf10\u300130\u3001100 kDa MWCO\uff09\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u306f\u3088\u304f\u4fdd\u6301\u3055\u308c\u3001\u6db2\u4f53\u306f\u5438\u53ce\u3055\u308c\u305a\u306b\u901a\u904e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u6db2\u4f53\u306e\u56de\u53ce\u7387\u306f\u226596%\u3092\u8a87\u308a\u307e\u3059\u3002 Big Tuna\u306fLNP\u306e\u8fc5\u901f\u306a\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3001\u6fc3\u7e2e\u3001\u4ea4\u63db\u306e\u305f\u3081\u306b\u3001\u9650\u5916\u6ffe\u904e\u6cd5\/\u30c0\u30a4\u30a2\u30d5\u30a3\u30eb\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\uff08UF\/DF\uff09\u3092\u4f7f\u7528\u3057\u3066\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3092\u5411\u4e0a\u3055\u305b\u308b\u5b8c\u5168\u81ea\u52d5\u5316\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u7a3c\u50cd\u4e2d\u306e\u30b5\u30f3\u30d7\u30eb\u91cf\u3092\u30e2\u30cb\u30bf\u30fc\u3057\u3001\u308d\u904e\u3055\u308c\u305f\u91cf\u3092\u623b\u3057\u3001\u6bce\u56de\u9069\u5207\u306a\u6bd4\u7387\u306e\u4ea4\u63db\u3068\u6fc3\u7e2e\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002LNP\u306e\u6709\u6a5f\u6eb6\u5a92\u306f\u901a\u5e38\u6025\u901f\u306b\u6d41\u308c\u307e\u3059\u304c\u3001Big Tuna\u306f\u9069\u5207\u306a\u8abf\u6574\u3092\u884c\u3046\u80fd\u529b\u3092\u5099\u3048\u3066\u3044\u308b\u306e\u3067\u3001\u30a6\u30a7\u30eb\u304c\u67af\u6e07\u3059\u308b\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u3002 Big Tuna\u3067\u306f\u3001Unfilter\u3068\u3044\u3046\u30d7\u30ec\u30fc\u30c8\u30d9\u30fc\u30b9\u306e\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u3059\u3079\u3066\u306eUnfilter\u306f\u518d\u751f\u30bb\u30eb\u30ed\u30fc\u30b9\u819c\u3092\u4f7f\u7528\u3057\u3066\u304a\u308a\u3001\u975e\u7279\u7570\u7684\u7d50\u5408\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u6700\u9069\u3067\u3059\u300224\u30a6\u30a7\u30eb\uff080.45\uff5e8mL\uff09\u306896\u30a6\u30a7\u30eb\uff08100\uff5e450\u03bcL\uff09\u306e2\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u3002Unfilter96\u306f\u30013\u300110\u300130\u3001100kDa\u306e\u5206\u5b50\u91cf\u30ab\u30c3\u30c8\u30aa\u30d5\uff08MWCO\uff09\u306b\u5bfe\u5fdc\u3057\u3001Unfilter24\u306f10\u300130\u3001100kDa\u306eMWCO\u306b\u5bfe\u5fdc\u3067\u304d\u307e\u3059\u300224\u30a6\u30a7\u30eb\u3001100kDa\u306eUnfilter\u306f\u3001\u5927\u91cf\u306eLNP\u30b5\u30f3\u30d7\u30eb\u3092\u9ad8\u901f\u3067\u51e6\u7406\u3059\u308b\u306e\u306b\u6700\u9069\u3067\u3059\u3002 \u5b9f\u8a3c Unagi\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u304a\u3088\u3073\u6fc3\u7e2e\u30d1\u30e9\u30e1\u30fc\u30bf\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5b8c\u5168\u6027\u3092\u4fdd\u3061\u306a\u304c\u3089\u3001\u9ad8\u3044\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u7387\u3092\u9054\u6210\u3059\u308b\u305f\u3081\u306b\u6700\u9069\u5316\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002LNP\u30b5\u30f3\u30d7\u30eb\u6e96\u5099\u306b\u304a\u3044\u3066\u3001\u30b5\u30a4\u30ba\u5909\u5316\u3084\u85ac\u7269\u5c01\u5165\u52b9\u7387\u306e\u3088\u3046\u306a\u30b5\u30f3\u30d7\u30eb\u306e\u54c1\u8cea\u3092\u72a0\u7272\u306b\u3059\u308b\u5fc5\u8981\u306a\u304f\u3001\u624b\u9593\u3092\u7bc0\u7d04\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 Big Tuna\u306f\u3001\u30da\u30a4\u30ed\u30fc\u30c9\u306e\u85ac\u7269\u5c01\u5165\u3092\u7dad\u6301\u3057\u306a\u304c\u3089\u30ca\u30ce\u7c92\u5b50\u306e\u6fc3\u7e2e\u3068\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3092\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5178\u578b\u7684\u306a\u30ca\u30ce\u7c92\u5b50\u306e\u6fc3\u7e2e\u3068\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3067\u3057\u305f\u3089\u3001\u6e96\u5099\u6642\u9593\u306f30\u5206\u672a\u6e80\u3067\u3001\u30e9\u30f3\u306f\u6700\u77ed90\u5206\u3067\u7d42\u4e86\u3067\u304d\u307e\u3059\u3002 Unagi \u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u3068\u6fc3\u7e2e\u306f\u3001\u5c11\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u3042\u3063\u3066\u3082\u5fc5\u305a\u884c\u308f\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u7169\u96d1\u306a\u4f5c\u696d\u3067\u3059\u3002\u6642\u9593\u304c\u304b\u304b\u308a\u3001\u76ee\u3092\u96e2\u305b\u305a\u3001\u7d42\u308f\u3063\u305f\u3068\u601d\u3063\u305f\u3089\u307e\u305f\u5fae\u8abf\u6574\u3092\u3057\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002Unagi\u3092\u4f7f\u3048\u3070\u3001\u30e9\u30dc\u306b\u5f35\u308a\u4ed8\u304f\u3053\u3068\u306a\u304f\u3001\u30e9\u30f3\u306e\u9593\u306f\u4ed6\u306e\u5834\u6240\u3067\u4f5c\u696d\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 UNAGI\u95a2\u9023\u60c5\u5831 Big Tuna Big 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